Indocyanine green (ICG) is capable of inducing a photothermal effect and the production of cytotoxic reactive oxygen species for cancer therapy. However, the major challenge in applying ICG molecules for antitumor therapy is associated with their instability in aqueous conditions and rapid clearance from blood circulation, which causes insufficient bioavailability at the tumor site. Herein, we conjugated ICG molecules with Prussian blue nanoparticles enclosing a Fe₃O₄ nanocore, which was facilitated by cationic polyethyleneimine via electrostatic adsorption. The nanocarrier-loaded ICG formed stable aggregates that enhanced cellular uptake and prevented fluorescence quenching. Moreover, the strong superparamagnetism of the Fe₃O₄ core in the obtained nanocomposites further improved cellular internalization of the drugs guided by a localized magnetic field. The therapeutic efficacy of this nanoplatform was evaluated using tumor models established in nude mice, which demonstrated remarkable tumor ablation in vivo due to strong photothermal/photodynamic effects. This study provides promising evidence that this multifunctional nanoagent might function as an efficient mediator for combining photothermal and photodynamic cancer therapy.

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吲哚菁绿（ICG）能够诱导光热效应并产生用于癌症治疗的细胞毒性活性氧。但是，将ICG分子用于抗肿瘤治疗的主要挑战在于其在水性条件下的不稳定性以及迅速从血液循环中清除，这导致肿瘤部位的生物利用度不足。在本文中，我们将ICG分子与普鲁士蓝纳米颗粒共轭，该纳米颗粒包裹了Fe ₃ O ₄纳米核，这是通过阳离子聚乙烯亚胺通过静电吸附促进的。载有纳米载体的ICG形成稳定的聚集体，可增强细胞摄取并防止荧光猝灭。而且，Fe ₃ O ₄具有很强的超顺磁性所获得的纳米复合材料的核心部分进一步改善了药物在局部磁场作用下的细胞内在化。使用在裸鼠中建立的肿瘤模型评估了该纳米平台的治疗效果，由于强的光热/光动力作用，该模型在体内表现出显着的肿瘤消融作用。这项研究提供了有前途的证据，证明这种多功能纳米剂可以作为光热和光动力癌症治疗相结合的有效介质。

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