We have recently found significant variation in stroke vulnerability among substrains of C57BL/6 mice, observing that commonly used N-lineage substrains exhibit larger infarcts than C57BL/6J and related substrains. Parallel variation was also seen with respect to sex differences in stroke vulnerability, in that C57BL/6 mice of the N-lineage exhibited comparable infarct sizes in males and females, whereas infarcts tended to be smaller in females than in males of J-lineage substrains. This adds to the growing list of recognized phenotypic and genetic differences among C57BL/6 substrains. Although no previous studies have explicitly compared substrains with respect to sex differences in stroke vulnerability, unrecognized background mismatch has occurred in some studies involving control and genetically modified mice. The aims of this review are to: present the evidence for associated substrain- and sex-dependent differences in a mouse permanent occlusion stroke model; examine the extent to which the published literature in other models compares with these recent results; and consider the potential impact of unrecognized heterogeneity in substrain background on the interpretation of studies investigating the impact of genetic modifications on sex differences in stroke outcome. Substrain emerges as a critical variable to be documented in any experimental stroke study in mice.

中文翻译：

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C57BL / 6子菌株对小鼠卒中模型中性别依赖性差异的影响。

最近，我们发现C57BL / 6小鼠亚型在中风易感性方面存在显着差异，观察到常用的N谱系亚型比C57BL / 6J和相关亚型表现出更大的梗塞灶。在中风脆弱性方面的性别差异方面也观察到平行变化，因为N谱系的C57BL / 6小鼠在雄性和雌性中表现出可比的梗死面积，而雌性的梗死倾向小于J谱系亚种的雄性。 。这增加了C57BL / 6子菌株之间公认的表型和遗传差异的清单。尽管以前没有研究明确比较亚菌株在中风脆弱性方面的性别差异，但在一些涉及对照和转基因小鼠的研究中，发生了无法识别的背景不匹配现象。这次审查的目的是：提供有关小鼠永久性闭塞性卒中模型相关的亚应变和性别依赖性差异的证据；检查其他模型中已发表的文献与这些最新结果进行比较的程度；并考虑了亚种背景中无法识别的异质性对研究的解释的潜在影响，这些研究调查了遗传修饰对中风结局中性别差异的影响。在任何小鼠中风的实验研究中，亚菌株都作为关键变量出现。并考虑了亚种背景中无法识别的异质性对研究的解释的潜在影响，这些研究调查了遗传修饰对中风结局中性别差异的影响。在任何小鼠中风实验研究中，亚菌株都作为关键变量出现。并考虑亚变种背景中无法识别的异质性对研究的解释的潜在影响，这些研究调查了遗传修饰对中风结局中性别差异的影响。在任何小鼠中风实验研究中，亚菌株都作为关键变量出现。