Despite the importance of 2-phenylacetic acid, a plant hormone in the endogenous auxin family, its biosynthesis pathway has yet to be elucidated. In this study, we developed a novel whole-cell biosensor for the simultaneous quantification of 2-phenylacetic acid (PA) and 2-phenylethylamine (PEA) through the regulation of bacterial catabolism of aromatic compounds. We used the PA regulon to enable the recognition of PA and PEA. Differentiation of PEA from PA involves the incorporation of the FeaR regulon within the same whole-cell biosensor to report the presence of aromatic amines. The proposed system is highly sensitive to PA as well as PEA.

中文翻译：

---

具有双信号输出的基于细胞的生物传感器，可同时定量苯乙酸和苯乙胺

尽管2-苯基乙酸是内源性生长素家族中的一种植物激素，但其生物合成途径尚未阐明。在这项研究中，我们开发了一种新型的全细胞生物传感器，可通过调节芳香族化合物的细菌分解代谢来同时定量2-苯基乙酸（PA）和2-苯基乙胺（PEA）。我们使用PA调节剂来识别PA和PEA。PEA与PA的区别涉及在同一全细胞生物传感器中掺入FeaR regulon以报告芳族胺的存在。拟议的系统对PA和PEA高度敏感。