Aluminum (Al) is widespread in the environment including the ocean. The effects of Al on marine organisms have attracted more and more attention in recent years. However, the mechanisms of uptake of Al by marine organisms and the subcellular distribution of Al once assimilated are unknown. Here we report the uptake and subcellular distribution of Al in a marine diatom Thalassiosira weissflogii. Short-term (< 120 min) uptake experiments showed that the Al uptake rate by the diatom was 0.033 ± 0.013 fmol^-1 cell^-1 min^-1 (internalization flux normalized to the exposure Al concentration of 2 µM = 0.034 ± 0.013 nmol m⁻² min⁻¹ nM⁻¹). Subcellular fractionation experiments showed that the internalized Al was partitioned to subcellular components in the following order: granules (69 ± 5%) > debris (17 ± 4%) > organelles (12 ± 2%) > heat-stable peptides (HSP) (~2%) > heat-denaturable proteins (HDP) (< 1%), indicating that the majority of intracellular Al was detoxified and stored in inorganic forms. The subcellular distribution of Al in the diatom is different from that of Al in freshwater green algae, in which most of the internalized Al is partitioned to organelles. We also evaluated an artificial seawater-based EDTA rinse solution to remove Al adsorbed on the diatom cell surface. Overall, our study provides new information to understand the mechanisms of uptake of Al by marine diatoms, and the mechanisms responsible for the biological effects (both toxic and beneficial) of Al on the growth of marine phytoplankton, especially diatoms.

铝（Al）广泛存在于包括海洋在内的环境中。近年来，铝对海洋生物的影响已引起越来越多的关注。然而，海洋生物对铝的吸收机制以及一旦被吸收后铝的亚细胞分布是未知的。在这里，我们报告海洋硅藻Thalassiosira weissflogii中铝的吸收和亚细胞分布。短期（<120分钟）吸收实验表明，硅藻对Al的吸收速率为0.033±0.013 fmol ^-1细胞^-1 min ^-1（内化通量标准化为2 AlM的暴露Al浓度= 0.034±0.013 nmol m ⁻² min ^-1 nM ^-1）。亚细胞分级分离实验表明，内在化的Al按以下顺序分配到亚细胞成分中：颗粒（69±5％）>碎片（17±4％）>细胞器（12±2％）>热稳定肽（HSP）（ （〜2％）>热变性蛋白（HDP）（<1％），表明大多数细胞内铝被解毒并以无机形式存储。Al在硅藻中的亚细胞分布与淡水绿藻中的Al亚细胞分布不同，在Alwater中，大部分内在化的Al被分配到细胞器中。我们还评估了一种人工海水基EDTA漂洗溶液，以去除硅藻细胞表面吸附的Al。总体而言，我们的研究提供了新的信息，以了解海洋硅藻吸收Al的机制，