Highlights • DamID adaptation to primary hepatocytes may preserve tissue 3D genome architecture.• Growth factors, vector tropism and enhancers are needed for DamID in primary cells.• Mitochondrial contamination can yield high background signal in primary cells.• Signal intensity comparisons can increase calling of interesting differential LADs.

中文翻译：

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用于小鼠肝细胞原代培养的 DamID 优化

亮点 • DamID 适应原代肝细胞可以保留组织 3D 基因组结构。• 原代细胞中的 DamID 需要生长因子、载体趋向性和增强剂。• 线粒体污染会在原代细胞中产生高背景信号。• 信号强度比较可以增加对原代细胞的调用有趣的差分法援署。