Reported herein is a fluorescence assay for the rapid screening of metallo-β-lactamase (MBL) inhibitors. This assay employs a fluorogenic carbapenem CPC-1 as substrate and is compatible with all MBLs, including B1, B2 and B3 subclass MBLs. The efficiency of this assay was demonstrated by the rapid inhibition screening of a number of molecules against B2 MBL CphA and 2,3-dimercaprol was identified as a potent CphA inhibitor.

中文翻译：

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基于碳青霉烯的荧光测定法筛选金属β-内酰胺酶抑制剂

本文报道了用于快速筛选金属-β-内酰胺酶（MBL）抑制剂的荧光测定法。该测定法采用荧光碳青霉烯CPC-1作为底物，并且与所有MBL（包括B1，B2和B3亚类MBL）兼容。通过对B2 MBL CphA的许多分子进行快速抑制筛选，证明了该测定的效率，并确定了2,3-二聚己醇为有效的CphA抑制剂。