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Closed-loop all-optical interrogation of neural circuits in vivo.
Nature Methods ( IF 36.1 ) Pub Date : 2018-11-12 , DOI: 10.1038/s41592-018-0183-z
Zihui Zhang 1, 2 , Lloyd E Russell 1 , Adam M Packer 1, 3 , Oliver M Gauld 1 , Michael Häusser 1
Affiliation  

Understanding the causal relationship between neural activity and behavior requires the ability to perform rapid and targeted interventions in ongoing activity. Here we describe a closed-loop all-optical strategy for dynamically controlling neuronal activity patterns in awake mice. We rapidly tailored and delivered two-photon optogenetic stimulation based on online readout of activity using simultaneous two-photon imaging, thus enabling the manipulation of neural circuit activity ‘on the fly’ during behavior.



中文翻译:


体内神经回路的闭环全光学询问。



了解神经活动和行为之间的因果关系需要能够对正在进行的活动进行快速且有针对性的干预。在这里,我们描述了一种闭环全光学策略,用于动态控制清醒小鼠的神经元活动模式。我们基于使用同时双光子成像在线读出活动,快速定制并提供双光子光遗传学刺激,从而能够在行为过程中“动态”操纵神经回路活动。

更新日期:2018-12-10
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