A superamphiphobic surface composed of two different size ranges of TiO₂ nanoparticles was simply fabricated through spraying the perfluorosilane coated TiO₂ nanoparticles suspension dispersing in ethanol. The surface chemistry was finely regulated through gradient UV irradiation-induced organic compound degradation to fabricate surface with gradient solid surface energy or wettability. The fabricated surface shows good droplet sorting ability, which can successfully discriminate ethanol droplets with different concentrations. As a proof-of-concept, the biosensor application of this surface was demonstrated by using it for naked-eye ATP detection. Liquid droplets with different concentrations of ATP after ATP-dependent rolling circle amplification (RCA) can be effectively sorted by the surface. This developed biosensor methodology based on droplet sorting ability of the fabricated surface is energy-efficient and economical which is promising for biosensors, point-of-care testing, and biochemical assays.

中文翻译：

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可调节的超两性表面：裸眼ATP检测的平台

通过喷涂全氟硅烷涂覆的TiO _2可以简单地制造由两个不同尺寸范围的TiO ₂纳米颗粒组成的超疏水表面纳米颗粒悬浮液分散在乙醇中。通过梯度紫外线辐照引起的有机化合物降解来精细调节表面化学性质，以制造具有梯度固体表面能或润湿性的表面。制成的表面具有良好的液滴分选能力，可以成功地区分不同浓度的乙醇液滴。作为概念验证，通过将其用于裸眼ATP检测证明了该表面的生物传感器应用。经过ATP依赖性滚环扩增（RCA）后，具有不同浓度ATP的液滴可以通过表面有效地分类。这种基于制造表面的液滴分选能力而开发的生物传感器方法学，具有高能效和经济性，有望用于生物传感器，即时检验，