Abstract In the present study, we report the effects of substituting casein-stabilized hydrogenated coconut oil-in-water emulsions with high-emulsifying β-conglycinin (7S) and reduced-glycinin soy protein (RG) in the preparation of oil powders. The effects of monoglycrides (MG) and protein concentration on the interfacial shear rheological behavior and the encapsulation of 7S and RG were investigated. The mean droplet size of emulsions indicated that at the same protein content, the addition of MG significantly improved the emulsifying properties of 7S (mean droplet size decreased from 332 nm to 281 nm) but had negative effects on the emulsifying properties of RG (mean droplet size increased from 741 nm to 2285 nm). The oil powders formed by 7S appeared to aggregate, which indicated that protein denaturation occurred during spray drying. However, the emulsions and oil powders formed by RG in the absence of MG exhibited small particles and a favorable dispersibility without aggregation. A competitive effect of MG on the interfacial adsorption of RG was further confirmed by the interfacial shear rheological measurements. This study suggested that RG alone is a promising substitute for caseins in the production of oil powders, and also provided a novel perspective on the design of soy protein-based encapsulation systems.

中文翻译：

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单酸甘油酯对大豆蛋白包封和界面剪切流变行为的协同和竞争作用

摘要 在本研究中，我们报告了用高乳化 β-伴大豆球蛋白 (7S) 和还原大豆球蛋白 (RG) 代替酪蛋白稳定的氢化椰子油水乳剂制备油粉的效果。研究了单酸甘油酯 (MG) 和蛋白质浓度对界面剪切流变行为和 7S 和 RG 包封的影响。乳液的平均液滴尺寸表明，在蛋白质含量相同的情况下，MG的加入显着提高了7S的乳化性能（平均液滴尺寸从332 nm减小到281 nm），但对RG（平均液滴尺寸）的乳化性能有负面影响尺寸从 741 nm 增加到 2285 nm）。7S形成的油粉出现聚集现象，表明喷雾干燥过程中发生了蛋白质变性。然而，RG在没有MG的情况下形成的乳液和油粉表现出小颗粒和良好的分散性，没有聚集。界面剪切流变测量进一步证实了 MG 对 RG 界面吸附的竞争作用。这项研究表明，在油粉生产中，单独使用 RG 是酪蛋白的一种有前景的替代品，并且还为基于大豆蛋白的封装系统的设计提供了新的视角。