In the present study, mulberry oligosaccharides were produced from mulberry polysaccharide by physical ultrasonic hydrolysis, chemical acid hydrolysis and enzymatic hydrolysis. The impact of the oligosaccharides on the growth four probiotic bacteria—Lactobacillus plantarum, Leuconostoc mesenteroides, Lactobacillus casei and Lactobacillus rhamnosuswas examined. The oligosaccharides produced from β-mannanase hydrolysis had the greatest effect on the growth of L. rhamnosus (252% of the number of cells compared with medium without the hydrolyzate). The enzymatically-prepared mulberry oligosaccharides (EMPS) were superior to the untreated mulberry polysaccharide and commercially available prebiotics (isomaltooligosaccharides and galactooligosaccharides). L. rhamnosus was cultured with EMPS and then subjected to tolerance tests with artificial gastrointestinal fluids. Bacteria remained viable. Subsequently, simulated-digested L. rhamnosus cultured with EMPS was used in in vitro fermentation with human gut microbiota from fecal inoculum. After 48 h of the incubation, in the experimental group (with pretreated L. rhamnosus), the ratio of Bacteroidetes to Firmicutes was 0.848, compared with 0.659 in the control group (saline instead of L. rhamnosus), suggesting that L. rhamnosus and constituents in EMPS were capable of regulating the growth of intestinal microorganisms. Our results inform the potential for the development of mulberry oligosaccharides as a prebiotics or synbiotics.

在本研究中，通过物理超声水解，化学酸水解和酶促水解从桑poly多糖制得桑寡糖。检验了低聚糖对四种益生菌细菌的生长的影响：植物乳杆菌，间肠隐球菌，干酪乳杆菌和鼠李糖乳杆菌。β-甘露聚糖酶水解产生的寡糖对鼠李糖乳杆菌的生长影响最大（与没有水解产物的培养基相比，细胞数量的252％）。酶法制备的桑树寡糖（EMPS）优于未处理的桑树多糖和市售的益生元（异麦芽低聚糖和低聚半乳糖）。鼠李糖乳杆菌用EMPS培养，然后用人工胃肠液进行耐受性测试。细菌仍然可行。随后，用EMPS培养的模拟消化的鼠李糖乳杆菌与来自粪便接种物的人类肠道菌群一起用于体外发酵。孵育48小时后，在实验组中（用经预处理的鼠李糖乳杆菌），拟杆菌的比率为0.848，而对照组为0.659（盐水代替鼠李糖乳杆菌），这表明鼠李糖乳杆菌和EMPS中的成分能够调节肠道微生物的生长。我们的结果表明桑树寡糖作为益生元或合生元的潜力。