Abstract Nanofiber sponge, which showed high porosity and excellent blood compatibility, was successfully prepared by electrospinning and freeze-drying technology. The APTT (activated partial thromboplastin time) and PT (prothrombin time) of human plasma with nanofiber sponge were a little longer than control groups, which revealed the nanofiber sponge has excellent blood compatibility. By activating the carboxyl groups on the fibers, PMB (polymyxin B) was successfully immobilized on the nanofiber sponge. The adsorption performance was researched in different solutions. The adsorption capacity in physiological saline solution was around 17,889 EU/mL and the adsorption equilibrium time was about 40 min. The adsorption behavior not only obeyed to second-order kinetic equation, but also was conformed to Langmuir model, which turned out to be chemical adsorption and monolayer adsorption. The endotoxin removal rate in BSA (bovine serum albumin) physiological saline solution was about 96.6%, and the BSA recovery rate was around 75.6%, which indicated the PMB immobilized nanofiber sponge adsorbed endotoxin selectively. The endotoxin removal rate of normal nanofiber sponge was about 17.3%, while it was around 99.6% when it comes to PMB immobilized-nanofiber sponge, which manifested PMB played an important role in endotoxin adsorption. The endotoxin removal rate in human plasma reached 90%, and the adsorption reached equilibrium within 60 min, which proved the PMB immobilized-nanofiber sponge would have great potential for applying in clinical blood purification.

中文翻译：

---

多粘菌素B固定化纳米纤维海绵吸附内毒素

摘要 采用静电纺丝和冷冻干燥技术成功制备了具有高孔隙率和优良血液相容性的纳米纤维海绵。含有纳米纤维海绵的人血浆的APTT（活化部分凝血活酶时间）和PT（凝血酶原时间）比对照组稍长，这表明纳米纤维海绵具有优异的血液相容性。通过激活纤维上的羧基，PMB（多粘菌素 B）成功地固定在纳米纤维海绵上。研究了不同溶液中的吸附性能。在生理盐水溶液中的吸附容量约为 17,889 EU/mL，吸附平衡时间约为 40 min。吸附行为不仅服从二级动力学方程，而且符合朗缪尔模型，原来是化学吸附和单层吸附。BSA（牛血清白蛋白）生理盐水中内毒素去除率约为96.6%，BSA回收率约为75.6%，表明PMB固定化纳米纤维海绵选择性吸附内毒素。普通纳米纤维海绵的内毒素去除率约为17.3%，而PMB固定化纳米纤维海绵的内毒素去除率约为99.6%，说明PMB在内毒素吸附中起重要作用。人血浆中内毒素去除率达到90%，吸附在60 min内达到平衡，证明PMB固定化纳米纤维海绵在临床血液净化中具有很大的应用潜力。BSA（牛血清白蛋白）生理盐水中内毒素去除率约为96.6%，BSA回收率约为75.6%，表明PMB固定化纳米纤维海绵选择性吸附内毒素。普通纳米纤维海绵的内毒素去除率约为17.3%，而PMB固定化纳米纤维海绵的内毒素去除率约为99.6%，说明PMB在内毒素吸附中起重要作用。人血浆中内毒素去除率达到90%，吸附在60 min内达到平衡，证明PMB固定化纳米纤维海绵在临床血液净化中具有很大的应用潜力。BSA（牛血清白蛋白）生理盐水中内毒素去除率约为96.6%，BSA回收率约为75.6%，表明PMB固定化纳米纤维海绵选择性吸附内毒素。普通纳米纤维海绵的内毒素去除率约为17.3%，而PMB固定化纳米纤维海绵的内毒素去除率约为99.6%，说明PMB在内毒素吸附中起重要作用。人血浆中内毒素去除率达到90%，吸附在60 min内达到平衡，证明PMB固定化纳米纤维海绵在临床血液净化中具有很大的应用潜力。普通纳米纤维海绵的内毒素去除率约为17.3%，而PMB固定化纳米纤维海绵的内毒素去除率约为99.6%，说明PMB在内毒素吸附中起重要作用。人血浆中内毒素去除率达到90%，吸附在60 min内达到平衡，证明PMB固定化纳米纤维海绵在临床血液净化中具有很大的应用潜力。普通纳米纤维海绵的内毒素去除率约为17.3%，而PMB固定化纳米纤维海绵的内毒素去除率约为99.6%，说明PMB在内毒素吸附中起重要作用。人血浆中内毒素去除率达到90%，吸附在60 min内达到平衡，证明PMB固定化纳米纤维海绵在临床血液净化中具有很大的应用潜力。