当前位置: X-MOL 学术Anal. Bioanal. Chem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A MnO2 nanosheet-based ratiometric fluorescent nanosensor with single excitation for rapid and specific detection of ascorbic acid
Analytical and Bioanalytical Chemistry ( IF 3.8 ) Pub Date : 2018-11-07 , DOI: 10.1007/s00216-018-1439-2
Yanlong Lyu , Zhanhui Tao , Xiaodong Lin , Pengcheng Qian , Yunfei Li , Shuo Wang , Yaqing Liu

Ascorbic acid (AA) detection in biological sample and food sample is critical for human health. Herein, a MnO2 nanosheet (MnO2-NS)-based ratiometric fluorescent nanosensor has been developed for high sensitive and specific detection of AA. The MnO2-NS presents peroxidase-like activity and can oxidize non-fluorescent substrate of o-phenylenediamine (OPDA) into fluorescent substrate, presenting maximum fluorescence at 568 nm (F568). If MnO2-NS is premixed with AA, the MnO2-NS is then decomposed as Mn2+ by AA, decreasing the fluorescent intensity of F568. Meantime, AA is oxidized as dehydroascorbic acid (DHAA), which can react with OPDA to generate fluorescent substrate. A new fluorescence response is found at 425 nm (F425). The dual fluorescent responses can be excited with a universal excitation wavelength, simplifying the detection procedure. With F425/F568 as readout, limit of detection for AA reaches as low as 10.0 nM. Satisfactory recoveries are found for AA detection in serum and diverse beverages. The ratiometric strategy significantly eliminates false-negative and false-positive results, providing a cost-effective, rapid, and reliable way for AA detection in real sample.



中文翻译:

MnO2个纳米片的单激发比例荧光纳米传感器,用于快速,特异性地检测抗坏血酸

生物样品和食品样品中抗坏血酸(AA)的检测对人体健康至关重要。在本文中,已经开发了基于MnO 2纳米片(MnO 2 -NS)的比例荧光纳米传感器,用于AA的高灵敏度和特异性检测。MnO 2 -NS表现出类似过氧化物酶的活性,并且可以将苯二胺(OPDA)的非荧光底物氧化为荧光底物,从而在568 nm(F 568)处呈现最大荧光。如果将MnO 2 -NS与AA预混合,则MnO 2 -NS会被AA分解为Mn 2+,从而降低F 568的荧光强度。同时,AA被氧化为脱氢抗坏血酸(DHAA),可以与OPDA反应生成荧光底物。在425 nm(F 425)处发现了新的荧光响应。可以用通用的激发波长来激发双重荧光响应,从而简化了检测程序。使用F 425 / F 568作为读数,AA的检测限可低至10.0 nM。发现血清和各种饮料中的AA检测均具有令人满意的回收率。比率测量策略可显着消除假阴性和假阳性结果,为实际样品中的AA检测提供了一种经济高效,快速而可靠的方法。

更新日期:2018-11-07
down
wechat
bug