In-vitro antidiabetic and antihypertensive properties of novel camel skin gelatin hydrolysates (CSGHs) were reported for the first time. Effect of different proteolytic enzymes, time of hydrolysis and enzyme: substrate (E/S) ratio on the bioactive (antidiabetic and antihypertensive) properties was explored. Results revealed that CSGHs exhibited highly potent inhibitory activity against dipeptidyl peptidase-IV (DPP-IV), pancreatic α-amylase (PA) and angiotensin-I converting enzyme (ACE) compared to non-hydrolyzed camel skin gelatin. All three enzyme combinations used produced highly potent PA inhibitory hydrolysates while for DPP-IV inhibitory property, protease and alcalase-protease (A/P) in combination produced more potent hydrolysates than alcalase generated hydrolysates. Moreover, alcalase and protease generated hydrolysates were highly active in inhibiting ACE activity in comparison to their combination (AP) (P < 0.05). Overall, no significant effect of E/S ratio and time of hydrolysis on production of DPP-IV, PA and ACE inhibitory peptides were observed. These findings suggest that both enzymes i.e. alcalase and protease alone or in combination were capable of producing camel skin gelatin hydrolysates with highly potent antidiabetic and antihypertensive properties. The present study presents the first report on the production of camel skin gelatin hydrolysates with strong in-vitro antidiabetic and antihypertensive potential. Future studies should be directed to identify the sequence of peptides responsibe for antidiabetic and antihypertensive activities.

体外首次报道了新型骆驼皮肤明胶水解物（CSGH）的抗糖尿病和降压特性。探索了不同的蛋白水解酶，水解时间和酶：底物（E / S）比率对生物活性（抗糖尿病和降压）性能的影响。结果表明，与未水解的骆驼皮肤明胶相比，CSGHs对二肽基肽酶-IV（DPP-IV），胰腺α-淀粉酶（PA）和血管紧张素-I转化酶（ACE）表现出高度有效的抑制活性。所使用的所有三种酶组合均产生强效的PA抑制水解产物，而就DPP-IV抑制特性而言，蛋白酶和碱性蛋白酶（A / P）组合产生的水解产物比碱性蛋白酶产生的水解产物更为有效。而且，与它们的组合（AP）相比，由alcalase和蛋白酶产生的水解产物在抑制ACE活性方面具有更高的活性（P <0.05）。总体而言，未观察到E / S比和水解时间对DPP-IV，PA和ACE抑制肽的产生的显着影响。这些发现表明，两种酶，即单独的alcalase和蛋白酶或组合酶，均能够产生具有高度有效的抗糖尿病和抗高血压特性的骆驼皮明胶水解产物。本研究提出了有关骆驼皮明胶水解物的生产的第一份报告。这些发现表明，两种酶，即单独的alcalase和蛋白酶或组合酶，均能够产生具有高度有效的抗糖尿病和抗高血压特性的骆驼皮明胶水解产物。本研究提出了关于骆驼皮明胶水解物的生产的第一份报告。这些发现表明，两种酶，即单独的alcalase和蛋白酶或组合酶，均能够产生具有高度有效的抗糖尿病和抗高血压特性的骆驼皮明胶水解产物。本研究提出了关于骆驼皮明胶水解物的生产的第一份报告。体外抗糖尿病和降压潜力。未来的研究应针对鉴定抗糖尿病和降压活性的肽序列。