Volatile organic compounds (VOCs) released from bacterial species have been reported as plant growth inducers. In this sense, Lactuca sativa was used as model vegetable to prospect the effects of 2-nonanone released by Bacillus sp. BCT9 at cellular and organ structure level, so we present preliminary results about the physiological effects. In this study, 2-day-old L. sativa were exposed to 2-nonanone for 10 days under two delivery systems: 1) 2-nonanone (abrupt delivery) and 2) 2-nonanone + lanolin (controlled delivery). The X-ray elemental microanalysis, scanning electron and confocal laser microscopies techniques were used to evaluate physiological changes “in vivo” conditions. The results indicated that 2-nonanone increased root and shoot length independently of 2-nonanone delivery system after 7 days of exposition. Additionally, 2-nonanone elicited the increase of anthocyanin and not affects chlorophyll content and electrolyte leakage percentage. The abrupt delivery elicited the increase of both length and density of root hair without causing changes in size of cell epidermis, while controlled delivery induced stomatal opening. Besides, 2-nonanone exposition did not modify the composition and distribution of carbon, nitrogen, phosphorus, potassium, and chlorine in the surface of plant tissue. The results suggested that 2-nonanone acts as a bacterial signal molecule to elicit changes related to root development without damaging the external morphology while epidermal cells at leaf level are not affected, suggesting that 2-nonanone can be an important tool to apply to vegetables.

据报道，细菌释放的挥发性有机化合物（VOC）可作为植物生长诱导剂。从这个意义上说，以生菜为模型蔬菜来探讨芽孢杆菌释放的2-壬酮的作用。 BCT9在细胞和器官结构水平上，因此我们提出了有关生理效应的初步结果。在这项研究中，2 日龄的苜蓿在两种递送系统下暴露于 2-壬酮 10 天：1) 2-壬酮（突然递送）和 2) 2-壬酮 + 羊毛脂（控制递送）。 X射线元素微量分析、扫描电子和共焦激光显微镜技术用于评估“体内”条件下的生理变化。结果表明，暴露 7 天后，2-壬酮增加了根和芽的长度，与 2-壬酮递送系统无关。此外，2-壬酮引起花青素的增加，但不影响叶绿素含量和电解质渗漏百分比。突然递送引起根毛长度和密度的增加，而不引起细胞表皮大小的变化，而受控递送则诱导气孔打开。此外，2-壬酮暴露并没有改变植物组织表面碳、氮、磷、钾、氯的组成和分布。结果表明，2-壬酮作为细菌信号分子，引发与根发育相关的变化，而不损害外部形态，而叶水平的表皮细胞不受影响，这表明2-壬酮可以成为应用于蔬菜的重要工具。