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Antimicrobial peptide based magnetic recognition elements and Au@Ag-GO SERS tags with stable internal standards: a three in one biosensor for isolation, discrimination and killing of multiple bacteria in whole blood†
Chemical Science ( IF 7.6 ) Pub Date : 2018-11-02 00:00:00 , DOI: 10.1039/c8sc04637a Kaisong Yuan 1, 2, 3, 4, 5 , Qingsong Mei 5, 6, 7, 8 , Xinjie Guo 1, 2, 3, 4, 5 , Youwei Xu 5, 9, 10, 11 , Danting Yang 5, 12, 13, 14, 15 , Beatriz Jurado Sánchez 16, 17, 18, 19, 20 , Bingbing Sheng 1, 2, 3, 4, 5 , Chusheng Liu 1, 2, 3, 4, 5 , Ziwei Hu 1, 2, 3, 4, 5 , Guangchao Yu 4, 5, 21 , Hongming Ma 4, 5, 21 , Hao Gao 1, 2, 3, 4, 5 , Christoph Haisch 22, 23, 24, 25 , Reinhard Niessner 22, 23, 24, 25 , Zhengjing Jiang 1, 2, 3, 4, 5 , Haibo Zhou 1, 2, 3, 4, 5
Chemical Science ( IF 7.6 ) Pub Date : 2018-11-02 00:00:00 , DOI: 10.1039/c8sc04637a Kaisong Yuan 1, 2, 3, 4, 5 , Qingsong Mei 5, 6, 7, 8 , Xinjie Guo 1, 2, 3, 4, 5 , Youwei Xu 5, 9, 10, 11 , Danting Yang 5, 12, 13, 14, 15 , Beatriz Jurado Sánchez 16, 17, 18, 19, 20 , Bingbing Sheng 1, 2, 3, 4, 5 , Chusheng Liu 1, 2, 3, 4, 5 , Ziwei Hu 1, 2, 3, 4, 5 , Guangchao Yu 4, 5, 21 , Hongming Ma 4, 5, 21 , Hao Gao 1, 2, 3, 4, 5 , Christoph Haisch 22, 23, 24, 25 , Reinhard Niessner 22, 23, 24, 25 , Zhengjing Jiang 1, 2, 3, 4, 5 , Haibo Zhou 1, 2, 3, 4, 5
Affiliation
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In this study, a new biosensor based on a sandwich structure has been developed for the isolation and detection of multiple bacterial pathogens via magnetic separation and SERS tags. This novel assay relies on antimicrobial peptide (AMP) functionalized magnetic nanoparticles as “capturing” probes for bacteria isolation and gold coated silver decorated graphene oxide (Au@Ag-GO) nanocomposites modified with 4-mercaptophenylboronic acid (4-MPBA) as SERS tags. When different kinds of bacterial pathogens are combined with the SERS tags, the “fingerprints” of 4-MPBA show corresponding changes due to the recognition interaction between 4-MPBA and different kinds of bacterial cell wall. Compared with the label-free SERS detection of bacteria, 4-MPBA here can be used as an internal standard (IS) to correct the SERS intensities with high reproducibility, as well as a Raman signal reporter to enhance the sensitivity and amplify the differences among the bacterial “fingerprints”. Thus, three bacterial pathogens (Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa) were successfully isolated and detected, with the lowest concentration for each of the strains detected at just 101 colony forming units per mL (CFU mL−1). According to the changes in the “fingerprints” of 4-MPBA, three bacterial strains were successfully discriminated using discriminant analysis (DA). In addition, the AMP modified Fe3O4NPs feature high antibacterial activities, and can act as antibacterial agents with low cellular toxicology in the long-term storage of blood for future safe blood transfusion applications. More importantly, this novel method can be applied in the detection of bacteria from clinical patients who are infected with bacteria. In the validation analysis, 97.3% of the real blood samples (39 patients) could be classified effectively (only one patient infected with E. coli was misclassified). The multifunctional biosensor presented here allows for the simultaneous isolation, discrimination and killing of bacteria, suggesting its high potential for clinical diagnosis and safe blood transfusions.
中文翻译：
基于抗菌肽的磁识别元件和具有稳定内标的Au @ Ag-GO SERS标签：三合一生物传感器，用于全血中多种细菌的分离，鉴别和杀灭†
在这项研究中，已开发出一种基于三明治结构的新型生物传感器，用于通过多种途径分离和检测多种细菌病原体。磁选和SERS标签。这种新颖的测定方法依赖于抗菌肽（AMP）官能化的磁性纳米颗粒，作为“捕获”探针用于细菌分离和镀金的修饰有4-巯基苯基硼酸（4-MPBA）作为SERS标签的镀银氧化石墨烯（Au @ Ag-GO）纳米复合材料。 。当不同种类的细菌病原体与SERS标签结合使用时，由于4-MPBA与不同种类细菌细胞壁之间的识别相互作用，4-MPBA的“指纹”显示出相应的变化。与无标记的细菌SERS检测相比，此处的4-MPBA可以用作内标（IS）以高再现性校正SERS强度，还可以使用拉曼信号报告仪来增强灵敏度并扩大之间的差异。细菌的“指纹”。因此，成功分离并检测到了大肠杆菌，金黄色葡萄球菌和铜绿假单胞菌，每种菌株的最低浓度仅为每毫升10 1个菌落形成单位（CFU mL -1）。根据4-MPBA“指纹”的变化，使用判别分析（DA）成功地辨别了三种细菌菌株。此外，AMP改性的Fe 3 O 4NPs具有很高的抗菌活性，在长期储存血液中可用作低细胞毒理学的抗菌剂，可用于将来安全的输血应用。更重要的是，这种新方法可用于检测感染了细菌的临床患者的细菌。在验证分析中，可以对97.3％的真实血液样本（39名患者）进行有效分类（只有一名感染了大肠杆菌的患者被错误分类）。这里展示的多功能生物传感器可以同时分离，区分和杀死细菌，这表明它在临床诊断和安全输血方面具有很高的潜力。
更新日期：2018-11-02
中文翻译：
基于抗菌肽的磁识别元件和具有稳定内标的Au @ Ag-GO SERS标签：三合一生物传感器，用于全血中多种细菌的分离，鉴别和杀灭†
在这项研究中，已开发出一种基于三明治结构的新型生物传感器，用于通过多种途径分离和检测多种细菌病原体。磁选和SERS标签。这种新颖的测定方法依赖于抗菌肽（AMP）官能化的磁性纳米颗粒，作为“捕获”探针用于细菌分离和镀金的修饰有4-巯基苯基硼酸（4-MPBA）作为SERS标签的镀银氧化石墨烯（Au @ Ag-GO）纳米复合材料。 。当不同种类的细菌病原体与SERS标签结合使用时，由于4-MPBA与不同种类细菌细胞壁之间的识别相互作用，4-MPBA的“指纹”显示出相应的变化。与无标记的细菌SERS检测相比，此处的4-MPBA可以用作内标（IS）以高再现性校正SERS强度，还可以使用拉曼信号报告仪来增强灵敏度并扩大之间的差异。细菌的“指纹”。因此，成功分离并检测到了大肠杆菌，金黄色葡萄球菌和铜绿假单胞菌，每种菌株的最低浓度仅为每毫升10 1个菌落形成单位（CFU mL -1）。根据4-MPBA“指纹”的变化，使用判别分析（DA）成功地辨别了三种细菌菌株。此外，AMP改性的Fe 3 O 4NPs具有很高的抗菌活性，在长期储存血液中可用作低细胞毒理学的抗菌剂，可用于将来安全的输血应用。更重要的是，这种新方法可用于检测感染了细菌的临床患者的细菌。在验证分析中，可以对97.3％的真实血液样本（39名患者）进行有效分类（只有一名感染了大肠杆菌的患者被错误分类）。这里展示的多功能生物传感器可以同时分离，区分和杀死细菌，这表明它在临床诊断和安全输血方面具有很高的潜力。
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