Bisphenol A (BPA) is one of the endocrine-disrupting chemicals which might cause reproductive and endocrine system diseases, and poses a serious threat to the ecosystem and human health. This paper reports an ultrasensitive sensor for trace BPA detection employing fluorescence resonance energy transfer (FRET) between modified upconversion nanoparticles (UCNPs) and tetramethylrhodamine. To circumvent the problems of low luminous efficiency of FRET and low sensitivity of sensor, the upconversion nanoparticles with very strong fluorescence efficiency were prepared and quantitatively modified. Results showed that the concentrations of amino groups and streptavidin were 43 nmol/mg and 6.12 μg/mg on the surface of the UCNPs, respectively. Under the optimal detection conditions, the peak intensity of UCNPs at 547 nm was linear with the logarithm of the BPA concentration with the detection limit of 0.05 ng/mL. Without complicated pre-processing, the recoveries were in general between 91.0 and 115.0% in tap water, river water, and disposable paper cup water. Therefore, the proposed sensor is suitable for effective sensing of trace BPA in water samples.

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双酚A（BPA）是破坏内分泌的化学物质之一，可能导致生殖和内分泌系统疾病，对生态系统和人类健康构成严重威胁。本文报道了一种用于痕量BPA检测的超灵敏传感器，该传感器在修饰的上转换纳米颗粒（UCNPs）和四甲基罗丹明之间使用了荧光共振能量转移（FRET）。为了解决FRET发光效率低，传感器灵敏度低的问题，制备了荧光强度非常高的上转换纳米粒子并进行了定量修饰。结果显示，在UCNP表面上，氨基和链霉亲和素的浓度分别为43nmol / mg和6.12μg/ mg。在最佳检测条件下，547 nm处的UCNPs峰强度与BPA浓度的对数呈线性关系，检出限为0.05 ng / mL。如果不进行复杂的预处理，自来水，河水和一次性纸杯水的回收率通常在91.0％至115.0％之间。因此，所提出的传感器适用于有效检测水样中的痕量BPA。

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