Pore Engineering for Enhanced Mass Transport in Encapsulin Nanocompartments,ACS Synthetic Biology

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Pore Engineering for Enhanced Mass Transport in Encapsulin Nanocompartments
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2018-10-30 00:00:00 , DOI: 10.1021/acssynbio.8b00295
Elsie M. Williams ₁ , Se Min Jung ₁ , Jennifer L. Coffman ₁ , Stefan Lutz ₁

Affiliation

Encapsulins are robust and engineerable proteins that form hollow, nanosized, icosahedral capsids, making them attractive vehicles for drug delivery, scaffolds for synthetic bionanoreactors, and artificial organelles. A major limitation of native encapsulins is the small size of pores in the protein shell. At 3 Å diameter, these pores impose significant restrictions on the molecular weight and diffusion rate of potential substrates. By redesigning the pore-forming loop region in encapsulin from Thermotoga maritima, we successfully enlarged pore diameter up to an estimated 11 Å and increased mass transport rates by 7-fold as measured by lanthanide ion diffusion assay. Our study demonstrates the high tolerance of encapsulin for protein engineering and has created a set of novel, functionally improved scaffolds for applications as bionanoreactors.

中文翻译：

增强胶囊蛋白纳米隔室中传质的孔工程

胶囊蛋白是结实的，可工程化的蛋白质，可形成空心的，纳米的二十面体衣壳，使其成为用于药物输送的有吸引力的载体，用于合成生物纳米反应器的支架和人造细胞器。天然胶囊蛋白的主要局限性是蛋白质壳中小孔的大小。直径为3Å时，这些孔对潜在底物的分子量和扩散速率施加了明显的限制。通过重新设计滨海嗜热菌中的胶囊蛋白中的孔形成环区域，我们成功地将孔径扩大到了估计的11Å，并且通过镧系元素离子扩散测定法测得的传质速率提高了7倍。我们的研究证明了荚膜蛋白对蛋白质工程的高耐受性，并创造了一套新颖的，功能上得到改进的支架，可用作生物纳米反应器。

更新日期：2018-10-30

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