当前位置: X-MOL 学术Nat. Methods › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
FLIRT: fast local infrared thermogenetics for subcellular control of protein function.
Nature Methods ( IF 36.1 ) Pub Date : 2018-10-30 , DOI: 10.1038/s41592-018-0168-y
Sophia M Hirsch 1 , Sriramkumar Sundaramoorthy 2, 3 , Tim Davies 2 , Yelena Zhuravlev 1 , Jennifer C Waters 4 , Mimi Shirasu-Hiza 1 , Julien Dumont 5 , Julie C Canman 2
Affiliation  

FLIRT (fast local infrared thermogenetics) is a microscopy-based technology to locally and reversibly manipulate protein function while simultaneously monitoring the effects in vivo. FLIRT locally inactivates fast-acting temperature-sensitive mutant proteins. We demonstrate that FLIRT can control temperature-sensitive proteins required for cell division, Delta-Notch cell fate signaling, and germline structure in Caenorhabditis elegans with cell-specific and even subcellular precision.

中文翻译:

FLIRT:快速局部红外热遗传学,用于蛋白质功能的亚细胞控制。

FLIRT(快速局部红外热遗传学)是一种基于显微镜的技术,可以局部和可逆地操纵蛋白质功能,同时监控体内的作用。FLIRT可局部灭活速效温度敏感突变蛋白。我们证明FLIRT可以控制特定的温度敏感蛋白,用于细胞分裂,Delta-Notch细胞命运信号传导和秀丽隐杆线虫的种系结构,具有细胞特异性甚至亚细胞精度。
更新日期:2018-12-10
down
wechat
bug