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Effect of Delivery Platforms Structure on the Epidermal Antigen Transport for Topical Vaccination
Biomacromolecules ( IF 5.5 ) Pub Date : 2018-10-30 00:00:00 , DOI: 10.1021/acs.biomac.8b01307 Ana S. Sonzogni 1 , Guy Yealland 2 , Mrityunjoy Kar 3 , Stefanie Wedepohl 3 , Luis M. Gugliotta 1 , Verónica D. G. Gonzalez 1 , Sarah Hedtrich 2 , Marcelo Calderón 3 , Roque J. Minari 1
Biomacromolecules ( IF 5.5 ) Pub Date : 2018-10-30 00:00:00 , DOI: 10.1021/acs.biomac.8b01307 Ana S. Sonzogni 1 , Guy Yealland 2 , Mrityunjoy Kar 3 , Stefanie Wedepohl 3 , Luis M. Gugliotta 1 , Verónica D. G. Gonzalez 1 , Sarah Hedtrich 2 , Marcelo Calderón 3 , Roque J. Minari 1
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Transdermal immunization is highly attractive because of the skin’s accessibility and unique immunological characteristics. However, it remains a relatively unexplored route of administration because of the great difficulty of transporting antigens past the outermost layer of skin, the stratum corneum. In this article, the abilities of three poly(N-vinylcaprolactam) (PVCL)-based thermoresponsive assemblies—PVCL hydrogels and nanogels plus novel film forming PVCL/acrylic nanogels—to act as protein delivery systems were investigated. Similar thermal responses were observed in all systems, with transition temperatures close to 32 °C, close to that of the skin surface. The investigated dermal delivery systems showed no evidence of cytotoxicity in human fibroblasts and were able to load and release ovalbumin (OVA), a well-studied antigen, in a temperature-dependent manner in vitro. The penetration of OVA into ex vivo human skin following topical application was evaluated, where enhanced skin delivery was seen for the OVA-loaded PVCL systems relative to administration of the protein alone. The distinct protein release and skin penetration profiles observed for the different PVCL assemblies were here discussed on the basis of their structural differences.
中文翻译：
传递平台结构对局部疫苗表皮抗原转运的影响
由于皮肤的可及性和独特的免疫学特性，经皮免疫非常吸引人。然而，由于将抗原转运通过皮肤的最外层即角质层非常困难，因此它仍然是相对未开发的给药途径。在本文中，三个poly（N研究了基于乙烯基己内酰胺（PVCL）的热响应组件-PVCL水凝胶和纳米凝胶，以及新型成膜的PVCL /丙烯酸纳米凝胶-充当蛋白质递送系统。在所有系统中观察到相似的热响应，转变温度接近32°C，接近皮肤表面的温度。所研究的皮肤递送系统没有显示出对人类成纤维细胞有细胞毒性的证据，并且能够在体外以温度依赖性方式负载和释放卵白蛋白（OVA），卵白蛋白（一种经过充分研究的抗原）。评估了局部施用后OVA对离体人皮肤的渗透性，其中与单独施用蛋白质相比，OVA-负载的PVCL体系的皮肤递送增强。
更新日期：2018-10-30
中文翻译：
传递平台结构对局部疫苗表皮抗原转运的影响
由于皮肤的可及性和独特的免疫学特性，经皮免疫非常吸引人。然而，由于将抗原转运通过皮肤的最外层即角质层非常困难，因此它仍然是相对未开发的给药途径。在本文中，三个poly（N研究了基于乙烯基己内酰胺（PVCL）的热响应组件-PVCL水凝胶和纳米凝胶，以及新型成膜的PVCL /丙烯酸纳米凝胶-充当蛋白质递送系统。在所有系统中观察到相似的热响应，转变温度接近32°C，接近皮肤表面的温度。所研究的皮肤递送系统没有显示出对人类成纤维细胞有细胞毒性的证据，并且能够在体外以温度依赖性方式负载和释放卵白蛋白（OVA），卵白蛋白（一种经过充分研究的抗原）。评估了局部施用后OVA对离体人皮肤的渗透性，其中与单独施用蛋白质相比，OVA-负载的PVCL体系的皮肤递送增强。
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