Biodegradable plastics have emerged as an encouraging alternative to reduce the production of plastic waste, especially for agricultural mulches. However, degradation of these plastics in the field may involve the release of products from the mulch into the soil, before and during the in-soil biodegradation. The present work aims to assess the potential effects on two agricultural plant species (Lactuca sativa L. –lettuce-, and Lycopersicon esculentum Mill. -tomato) of extracts from biodegradable (BDMs: Mater-Bi, Bioplast-SP4 and SP-6, BioFilm, BioFlex, Ecovio, Mirel, Paper) and Polyethylene mulch films. A previously designed highly sensitive in vitro ecotoxicity test was used.

Some of the extracts from the biodegradable plastics had effects on plant development. Germination was reduced by Bioplast films treatments, both in lettuce (B-SP4) and in tomato (B-SP4 and B-SP-6). In lettuce, root development was notably reduced by all treatments except for Paper and Polyethylene. Plant aerial growth was also limited with Bioplast and BioFlex, but enhanced with Paper extracts. At a whole, tomato plants showed higher sensitivity than lettuce in the test. Tomato aerial plant part and root growth were reduced by all treatments with the exception of BioFlex and Polyethylene. For both plant species, inhibitory effects on development were associated to proline increases, a physiological marker for some plant stresses.

It can be concluded that the contact of unused biodegradable films with a water solution may result in changes in plant development that depend on the nature of the biodegradable film. The in vitro used test revealed to be a highly sensitive tool for ecotoxicity studies. These results are to contribute to design safe materials for agricultural applications.

可生物降解的塑料已成为减少塑料废物（特别是农用覆盖物）生产的令人鼓舞的替代方法。然而，这些塑料在田间的降解可能涉及在土壤中生物降解之前和过程中，产品从覆盖物释放到土壤中。本工作的目的是评估在两个农业植物物种的潜在影响（莴L. -lettuce-，和番茄从可生物降解的（BDMS提取物的轧机-tomato）：母校碧，的Bioplast-SP4和SP-6， BioFilm，BioFlex，Ecovio，Mirel，Paper）和聚乙烯覆盖膜。使用先前设计的高度敏感的体外生态毒性测试。

一些可生物降解塑料的提取物对植物发育有影响。在生菜（B-SP4）和番茄（B-SP4和B-SP-6）中，均通过Bioplast膜处理减少了发芽。在生菜中，除纸和聚乙烯外，所有处理均显着降低了根的发育。Bioplast和BioFlex也限制了植物的气生生长，但纸提取物却增强了植物的气生生长。总体而言，在测试中，番茄植株比生菜具有更高的敏感性。除BioFlex和聚乙烯外，所有处理均降低了番茄空中植物部分和根系生长。对于这两种植物，对发育的抑制作用与脯氨酸的增加有关，脯氨酸是某些植物胁迫的生理标记。

可以得出结论，未使用的可生物降解膜与水溶液的接触可能导致植物发育的变化，这取决于可生物降解膜的性质。在体外使用的测试发现是对生态毒性研究的一个高度敏感的工具。这些结果有助于设计用于农业应用的安全材料。