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Aligned Chitosan-Gelatin Cryogel-Filled Polyurethane Nerve Guidance Channel for Neural Tissue Engineering: Fabrication, Characterization, and In Vitro Evaluation.
Biomacromolecules ( IF 5.5 ) Pub Date : 2018-11-02 , DOI: 10.1021/acs.biomac.8b01308 Anamika Singh 1 , Parvaiz A Shiekh 1 , Mainak Das 1 , Jukka Seppälä 2 , Ashok Kumar 1, 2, 3
Biomacromolecules ( IF 5.5 ) Pub Date : 2018-11-02 , DOI: 10.1021/acs.biomac.8b01308 Anamika Singh 1 , Parvaiz A Shiekh 1 , Mainak Das 1 , Jukka Seppälä 2 , Ashok Kumar 1, 2, 3
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Recent trends in peripheral nerve regeneration are directed toward the development of nerve guidance channels to assist the regeneration of the nerves across critical size defects. Advanced nerve guidance channels (aNGCs) should possess multifunctional properties to direct the axonal regeneration from proximal to distal end, allow the concentration of growth factors secreted by the injured nerve end, and attenuate the ingrowth of scar tissue at the site of injury. The design of the nerve guidance channel (NGC) is critical for providing the necessary topographical, chemotactic, as well as haptotactic cues for efficient nerve regeneration. In this study, we have designed and fabricated clinically relevant aNGCs comprising an antioxidant polyurethane (PUAO) conduit filled with aligned chitosan-gelatin (CG) cryogel filler for peripheral nerve regeneration. The effects of temperature, polymer concentration, and cross-linker concentration on the physicochemical properties of the CG cryogel filler were studied. The synthesized scaffolds were evaluated by scanning electron microscopy (SEM) and compression testing to obtain the matrix best suited to form the aNGC. The nanofibrous PUAO conduit was fabricated by electrospinning with a wall thickness of 114.16 ± 26.91 μm, which was filled with CG (1.2/6.4%)-aligned cryogel matrix to obtain the aNGCs. The aNGCs with 2.01 ± 0.04 mm internal diameter, 15 mm length, and internal CG filler with a pore diameter of 29.60 ± 9.83 μm were fabricated. The aNGCs were evaluated by SEM and in vitro neuronal culture for biocompatibility and cellular alignment. In vitro dorsal root ganglion cultures showed the aligned growth and cellular migration along the aligned pores of aNGCs. With this study, we conclude that this clinically relevant aligned porous aNGC will have a promising effect in repair and regeneration of peripheral nerve injuries.
中文翻译：
对齐的壳聚糖-明胶冷冻凝胶填充的聚氨酯神经引导通道用于神经组织工程：制备，表征和体外评估。
周围神经再生的最新趋势是针对神经引导通道的发展，以协助跨临界大小缺陷的神经再生。先进的神经引导通道（aNGC）应具有多功能特性，可指导轴突从近端到远端的再生，使受损神经末端分泌的生长因子集中，并减轻损伤部位疤痕组织的向内生长。神经引导通道（NGC）的设计对于提供必要的地形，趋化性和触觉暗示对于有效的神经再生至关重要。在这项研究中，我们设计和制造了与临床相关的aNGC，包括抗氧化剂聚氨酯（PUAO）导管，其中填充有对齐的壳聚糖-明胶（CG）冷冻凝胶填料，用于周围神经再生。研究了温度，聚合物浓度和交联剂浓度对CG冷冻凝胶填料理化性质的影响。合成的支架通过扫描电子显微镜（SEM）和压缩测试进行评估，以获得最适合形成aNGC的基质。纳米纤维PUAO导管是通过电纺丝制成的，其壁厚为114.16±26.91μm，并填充有CG（1.2 / 6.4％）取向的冷冻凝胶基质，以获得aNGC。制备了内径为2.01±0.04 mm，长度为15 mm的aNGC和孔径为29.60±9.83μm的内部CG填料。通过SEM和体外神经元培养评估aNGC的生物相容性和细胞排列。体外背根神经节培养物显示沿aNGC的对齐孔的对齐生长和细胞迁移。通过这项研究，我们得出结论，这种临床相关的对齐多孔aNGC将在修复和再生周围神经损伤中具有有希望的作用。
更新日期：2018-10-24
中文翻译：
对齐的壳聚糖-明胶冷冻凝胶填充的聚氨酯神经引导通道用于神经组织工程：制备，表征和体外评估。
周围神经再生的最新趋势是针对神经引导通道的发展，以协助跨临界大小缺陷的神经再生。先进的神经引导通道（aNGC）应具有多功能特性，可指导轴突从近端到远端的再生，使受损神经末端分泌的生长因子集中，并减轻损伤部位疤痕组织的向内生长。神经引导通道（NGC）的设计对于提供必要的地形，趋化性和触觉暗示对于有效的神经再生至关重要。在这项研究中，我们设计和制造了与临床相关的aNGC，包括抗氧化剂聚氨酯（PUAO）导管，其中填充有对齐的壳聚糖-明胶（CG）冷冻凝胶填料，用于周围神经再生。研究了温度，聚合物浓度和交联剂浓度对CG冷冻凝胶填料理化性质的影响。合成的支架通过扫描电子显微镜（SEM）和压缩测试进行评估，以获得最适合形成aNGC的基质。纳米纤维PUAO导管是通过电纺丝制成的，其壁厚为114.16±26.91μm，并填充有CG（1.2 / 6.4％）取向的冷冻凝胶基质，以获得aNGC。制备了内径为2.01±0.04 mm，长度为15 mm的aNGC和孔径为29.60±9.83μm的内部CG填料。通过SEM和体外神经元培养评估aNGC的生物相容性和细胞排列。体外背根神经节培养物显示沿aNGC的对齐孔的对齐生长和细胞迁移。通过这项研究，我们得出结论，这种临床相关的对齐多孔aNGC将在修复和再生周围神经损伤中具有有希望的作用。
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