CRISPR-Cas9 Facilitated Multiple-Chromosome Fusion in Saccharomyces cerevisiae,ACS Synthetic Biology

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CRISPR-Cas9 Facilitated Multiple-Chromosome Fusion in Saccharomyces cerevisiae
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2018-10-23 00:00:00 , DOI: 10.1021/acssynbio.8b00397
Yangyang Shao ₁ , Ning Lu _{1,

2} , Zhongjun Qin ₁ , Xiaoli Xue ₁

Affiliation

Eukaryotic cells usually contain multiple linear chromosomes. Recently, we artificially created a functional single-chromosome yeast via sequential two-chromosome fusion utilizing the high performance of the CRISPR-Cas9 system and homologous recombination in Saccharomyces cerevisiae. In this paper, we adapted this method for the simultaneous fusion of multiple chromosomes. We demonstrated the fusion of two, two-chromosome sets with a 75% positive rate and three-chromosome fusions with a 50% positive rate. We also found that by using an additional selection marker, the positive rate of two-chromosome fusions reached 100%. Due to the simplicity, efficiency, and portability of this method, we expect that it can be easily adapted for multiple-chromosome fusions in other organisms.

中文翻译：

CRISPR-Cas9促进酿酒酵母中的多染色体融合

真核细胞通常包含多个线性染色体。最近，我们利用CRISPR-Cas9系统的高性能和酿酒酵母中的同源重组，通过连续的两染色体融合人工创建了功能单一的酵母。在本文中，我们将这种方法用于多条染色体的同时融合。我们展示了融合率达75％的两个两染色体融合体和融合率50％的三染色体融合体。我们还发现，通过使用其他选择标记，两个染色体融合体的阳性率达到100％。由于此方法的简单性，效率和可移植性，我们希望它可以轻松地适用于其他生物体中的多染色体融合。

更新日期：2018-10-23

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