Hierarchical manganese dioxide nanoflowers enable accurate ratiometric fluorescence enzyme-linked immunosorbent assay†,Nanoscale

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Hierarchical manganese dioxide nanoflowers enable accurate ratiometric fluorescence enzyme-linked immunosorbent assay†
Nanoscale ( IF 5.8 ) Pub Date : 2018-10-23 00:00:00 , DOI: 10.1039/c8nr07096b
Lei Jiao _{1,

2,

3,

4} , Lianhua Zhang _{4,

5,

6,

7} , Wenwen Du _{1,

2,

3,

4} , He Li _{1,

2,

3,

4,

8} , Dingyu Yang _{1,

2,

3,

4} , Chengzhou Zhu _{9,

10,

11,

12,

13}

Affiliation

We first developed a green, mild and rapid method for the preparation of hierarchical manganese dioxide nanoflowers (MnO₂ NFs) as nanozymes with intrinsic oxidase-like activity using citric acid for the reduction of potassium permanganate. The open structure of MnO₂ NFs can lead to a larger specific surface area for improving the antibody loading amount and oxidase mimicking activity. In addition, carboxyl groups from the residual citric acid on the surface of MnO₂ NFs afford a good affinity with the antibody through an amidation coupling reaction, which does not need complex surface modification anymore. Then, a reliable ratiometric fluorescence nanozyme-linked immunosorbent assay towards C-reactive protein (CRP) was designed successfully based on the MnO₂ NFs. The high sensitivity and clinical feasibility of the presented methodology were demonstrated, which hold great promise in biomedical fields. Moreover, the mild and simple process for the synthesis of MnO₂ NFs will make good contributions to many other applications.

中文翻译：

分层的二氧化锰纳米花可实现精确的比例荧光酶联免疫吸附测定†

我们首先开发了一种绿色，温和且快速的方法，用于使用柠檬酸还原高锰酸钾来制备具有内在氧化酶样活性的分级二氧化锰纳米花（MnO ₂ NFs）作为具有内在氧化酶活性的纳米酶。MnO ₂ NFs的开放结构可导致更大的比表面积，从而提高抗体的负载量和氧化酶模拟活性。此外，MnO ₂ NFs表面残留柠檬酸中的羧基通过酰胺化偶联反应与抗体具有良好的亲和力，不再需要复杂的表面修饰。然后，基于MnO成功地设计了一种针对C反应蛋白（CRP）的可靠的比例荧光纳米酶联免疫吸附试验。_2个NF。结果表明，该方法具有很高的敏感性和临床可行性，在生物医学领域具有广阔的前景。而且，温和而简单的MnO₂ NFs合成方法将为许多其他应用做出很好的贡献。

更新日期：2018-10-23

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