Docosahexaenoic acid (DHA, 22:6) is a natural active compound that has raised considerable interest due to its several biological effects. In this work, effects of free DHA on the physicochemical properties of dipalmitoylphosphatidylcholine (DPPC) liposomes are investigated in terms of lipid membrane structure, by means of temperature-dependent zeta potential measurements, density studies and molecular dynamics simulations. Experimental results predict, in good agreement with simulations that DHA readily incorporates into DPPC liposomes, localizing at the lipid headgroup region. These data show that DHA induces changes in the lipid bilayer structure as well as in membrane fluidity.

二十二碳六烯酸（DHA，22：6）是一种天然活性化合物，由于其多种生物学作用而引起了人们的极大兴趣。在这项工作中，通过依赖于温度的Zeta电势测量，密度研究和分子动力学模拟，研究了游离DHA对二棕榈酰磷脂酰胆碱（DPPC）脂质体理化性质的影响。实验结果与模拟非常吻合地预测，DHA容易掺入DPPC脂质体中，位于脂质头基区域。这些数据表明，DHA引起脂质双层结构以及膜流动性的变化。