Abstract The present study aims to develop natural biopolymer bioactive edible films. Particularly, blend (F-Bl) and bilayer (F-Bi) films based on grey triggerfish skin gelatin (G) and agar (A), incorporated or not with vine leaves ethanolic extract (VLE), at 5 and 10 mg/mL, were prepared using the casting method. First, VLE presented an important antioxidant potential evaluated by the iron (Fe2+) chelating activity, ferric (Fe3+) reducing antioxidant power, β-carotene bleaching protection and DPPH•-radical scavenging activity. Furthermore, VLE presented interesting antibacterial and antifungal activities. On the other hand, the composite films were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR), physicochemical, and thermal analyses. SEM micrographs showed that agar and gelatin were compatible. Moreover, the mechanical properties results showed that F-Bl and F-Bi were mechanically stronger and more deformable than control films. This change in mechanical properties can be explained by the formation of a dense network after agar addition, due to the interactions with the gelatin matrix. After their incorporation into polymer network, VLE increased slightly the thermal stability, while it improved greatly the antioxidant activity of the composite films (gelatin and agar) in a dose dependent manner. Thus, results encourage the further use of gelatin/agar/VLE films as active food packaging material.

中文翻译：

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含有藤叶生物活性化合物的灰色触发鱼明胶/琼脂双层和混合膜的开发和表征

摘要 本研究旨在开发天然生物聚合物生物活性可食用薄膜。特别是基于灰色触发鱼皮明胶 (G) 和琼脂 (A) 的混合 (F-Bl) 和双层 (F-Bi) 薄膜，掺入或不掺入藤叶乙醇提取物 (VLE)，浓度为 5 和 10 毫克/毫升, 是使用浇铸方法制备的。首先，通过铁 (Fe2+) 螯合活性、三价铁 (Fe3+) 还原抗氧化能力、β-胡萝卜素漂白保护和 DPPH•-自由基清除活性评估 VLE 具有重要的抗氧化潜力。此外，VLE 表现出有趣的抗菌和抗真菌活性。另一方面，通过扫描电子显微镜 (SEM)、傅里叶变换红外 (FTIR)、物理化学和热分析对复合膜进行了表征。SEM显微照片显示琼脂和明胶是相容的。而且，机械性能结果表明 F-Bl 和 F-Bi 比对照薄膜具有更强的机械强度和更易变形。由于与明胶基质的相互作用，加入琼脂后形成致密的网络可以解释机械性能的这种变化。加入聚合物网络后，VLE 略微增加了热稳定性，同时以剂量依赖性方式大大提高了复合膜（明胶和琼脂）的抗氧化活性。因此，结果鼓励进一步使用明胶/琼脂/VLE 薄膜作为活性食品包装材料。由于与明胶基质的相互作用。加入聚合物网络后，VLE 略微增加了热稳定性，同时以剂量依赖性方式大大提高了复合膜（明胶和琼脂）的抗氧化活性。因此，结果鼓励进一步使用明胶/琼脂/VLE 薄膜作为活性食品包装材料。由于与明胶基质的相互作用。加入聚合物网络后，VLE 略微增加了热稳定性，同时以剂量依赖性方式大大提高了复合膜（明胶和琼脂）的抗氧化活性。因此，结果鼓励进一步使用明胶/琼脂/VLE 薄膜作为活性食品包装材料。