A simple Schiff base was prepared by mild condensation reaction between a coumarin fluorescent group and diaminomaleonitrile, and it could serve as an excellent fluorescent probe for fast detection of ClO⁻ with high selectivity and sensitivity. Along with addition of ClO⁻, this probe fleetingly showed noteworthy “turn-on” phenomenon accompanied by an increase of fluorescence intensity and the change of emission color from yellow to blue. This change of fluorescence is so significant that it can be observed by the naked-eye under a handheld ultraviolet light of 365 nm. However, other common reactive oxygen species exhibited no or very little fluorescent response under the same conditions. The limit of detection of this probe toward ClO⁻ had a sensitivity feature as low as 9.6 nM. On account of these excellent features of short response time, remarkable fluorescence and color signal changes, high sensitivity and selectivity, this probe was effectively used for the fluorescence detection of ClO⁻ in water samples. The values of the relative standard deviation were between 1.41% and 2.91%. More importantly, this probe displays excellent imaging capability in cytoplasm as well as very low cell toxicity and was unambiguously applied to image ClO⁻ in living cells.

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一个简单的席夫碱用香豆素荧光基团和二氨基之间温和的缩合反应制备，并且它可以作为用于快速检测CLO的优良荧光探针^-以高选择性和灵敏度。随着加成CLO的^- ，该探针飞快表明值得注意“接通”的现象伴随着增加的荧光强度和发光颜色的从黄色到蓝色的变化。荧光的这种变化是如此显着，以至于在365 nm的手持紫外光下，肉眼可以观察到。然而，在相同条件下，其他常见的活性氧物种则没有或只有很少的荧光响应。检测这种探头向CLO的极限^-具有低至9.6 nM的灵敏度。是考虑的响应时间短，这些优良的特性，显着的荧光和色度信号的变化，高灵敏度和选择性，被有效地用于此探针CLO的荧光检测^-水样英寸 相对标准偏差的值在1.41％和2.91％之间。更重要的是，在细胞质中该探针表现出优良的成像能力以及非常低的细胞毒性，并明确地应用于图像CLO ^-在活细胞。

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