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Pt-grown carbon nanofibers for enzymatic glutamate biosensors and assessment of their biocompatibility†
RSC Advances ( IF 3.9 ) Pub Date : 2018-10-19 00:00:00 , DOI: 10.1039/c8ra07766e
Noora Isoaho 1 , Emilia Peltola 1 , Sami Sainio 2 , Jari Koskinen 2 , Tomi Laurila 1
Affiliation  

Application-specific carbon nanofibers grown from Pt-catalyst layers have been shown to be a promising material for biosensor development. Here we demonstrate immobilization of glutamate oxidase on them and their use for amperometric detection of glutamate at two different potentials. At −0.15 V vs. Ag/AgCl at concentrations higher than 100 μM the oxygen reduction reaction severely interferes with the enzymatic production of H2O2 and consequently affects the detection of glutamate. On the other hand, at 0.6 V vs. Ag/AgCl enzyme saturation starts to affect the measurement above a glutamate concentration of 100 μM. Moreover, we suggest here that glutamate itself might foul Pt surfaces to some degree, which should be taken into account when designing Pt-based sensors operating at high anodic potentials. Finally, the Pt-grown and Ni-grown carbon nanofibers were shown to be biocompatible. However, the cells on Pt-grown carbon nanofibers had different morphology and formation of filopodia compared to those on Ni-grown carbon nanofibers. The effect was expected to be caused rather by the different fiber dimensions between the samples than the catalyst metal itself. Further experiments are required to find the optimal dimensions of CNFs for biological purposes.

中文翻译:


用于酶促谷氨酸生物传感器的铂生长碳纳米纤维及其生物相容性评估†



由铂催化剂层生长的特定应用碳纳米纤维已被证明是用于生物传感器开发的有前途的材料。在这里,我们展示了谷氨酸氧化酶在其上的固定化以及它们在两种不同电位下对谷氨酸进行安培检测的用途。在-0.15 V vs. Ag/AgCl 浓度高于100 μM 时,氧还原反应严重干扰H 2 O 2的酶促产生,从而影响谷氨酸的检测。另一方面,在 0.6 V vs. Ag/AgCl 酶饱和度开始影响谷氨酸浓度高于 100 μM 的测量。此外,我们在此建议,谷氨酸本身可能会在某种程度上污染铂表面,在设计在高阳极电位下工作的铂基传感器时应考虑到这一点。最后,铂生长和镍生长的碳纳米纤维被证明具有生物相容性。然而,与镍生长的碳纳米纤维上的细胞相比,铂生长的碳纳米纤维上的细胞具有不同的形态和丝状伪足的形成。预计该效应是由样品之间不同的纤维尺寸引起的,而不是由催化剂金属本身引起的。需要进一步的实验来找到用于生物学目的的 CNF 的最佳尺寸。
更新日期:2018-10-19
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