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Single Molecule DNA Resensing Using a Two‐Pore Device
Small ( IF 13.0 ) Pub Date : 2018-10-17 , DOI: 10.1002/smll.201801890 Yuning Zhang 1 , Xu Liu 2 , Yanan Zhao 2 , Jen-Kan Yu 2 , Walter Reisner 1 , William B. Dunbar 2
Small ( IF 13.0 ) Pub Date : 2018-10-17 , DOI: 10.1002/smll.201801890 Yuning Zhang 1 , Xu Liu 2 , Yanan Zhao 2 , Jen-Kan Yu 2 , Walter Reisner 1 , William B. Dunbar 2
Affiliation
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A nanofluidic device is presented that, enables independent sensing and resensing of a single DNA molecule translocating through two nanopores with sub‐micrometer spacing. The device concept is based upon integrating a thin nitride membrane with microchannels etched in borosilicate glass. Pores, coupled to each microchannel, are connected via a fluid‐filled half‐space on the device backside, enabling translocation of molecules across each pore in sequence. Critically, this approach allows for independent application of control voltage and measurement of trans‐pore ionic current at each of the two pores, leading to 1) controlled assessment of molecular time of flight, 2) voltage‐tuned selective molecule recapture, and 3) ability to acquire two correlated translocation signatures for each molecule analyzed. Finally, the rare cocapture of a single chain threading simultaneously through each of the two pores is reported.
中文翻译：
使用两孔设备进行单分子DNA还原
提出了一种纳米流体装置，该装置能够对单个DNA分子进行独立感测和重新传感，该分子通过亚微米间距穿过两个纳米孔。该设备的概念基于将氮化硅薄膜与在硼硅酸盐玻璃中蚀刻的微通道集成在一起。孔与每个微通道相连，通过设备背面的充满流体的半空间连接，从而使分子能够按顺序跨每个孔迁移。至关重要的是，该方法允许在两个孔中的每个孔上独立施加控制电压和测量跨孔离子电流，从而导致1）分子飞行时间的受控评估，2）电压调整的选择性分子捕获和3）能够为每个分析的分子获得两个相关的易位标记。最后，
更新日期：2018-10-17
中文翻译：
使用两孔设备进行单分子DNA还原
提出了一种纳米流体装置，该装置能够对单个DNA分子进行独立感测和重新传感，该分子通过亚微米间距穿过两个纳米孔。该设备的概念基于将氮化硅薄膜与在硼硅酸盐玻璃中蚀刻的微通道集成在一起。孔与每个微通道相连，通过设备背面的充满流体的半空间连接，从而使分子能够按顺序跨每个孔迁移。至关重要的是，该方法允许在两个孔中的每个孔上独立施加控制电压和测量跨孔离子电流，从而导致1）分子飞行时间的受控评估，2）电压调整的选择性分子捕获和3）能够为每个分析的分子获得两个相关的易位标记。最后，
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