Ultrasound-assisted extraction (UAE) was optimized for fast and effective isolation of blackthorn flower polyphenols. The effects of ethanol concentration (50% and 70%) in extraction solvent, ultrasound amplitude (50%, 75%, and 100%) and extraction time (3, 6, and 9 min) were evaluated. UPLC-MS2 was used to determine phenolic profile of blackthorn flowers extracts. A total of 28 different phenolic compounds were identified, belonging to the classes of hydroxycinnamic acids, flavonol glycosides and flavanols. Kaempferol and quercetin derivatives were the most abundant compounds represented by kaempferol-pentoside and rhamnoside in concentrations up to 494.94 and 436.62 mg/100 g and quercetin-pentoside in concentration up to 226.75 mg/100 g. Observed parameters of UAE did not affect the qualitative phenolic composition of blackthorn flower extracts. On the contrary, the concentration of polyphenols increased with higher ethanol concentration in solvent, higher ultrasound amplitude and prolongation of extraction time. The established conditions for optimal extraction of blackthorn flower polyphenols using ultrasound were: 70% ethanol as extraction solvent, 75% of ultrasound amplitude and 9 min extraction time, resulting in extract rich in polyphenols with a great potential for use in pharmaceutical and food industry. PRACTICAL APPLICATION: In this study, ultrasound-assisted extraction (UAE) was optimized in order to establish fast and effective method for production of crude blackthorn flower extract rich in polyphenols. UPLC-MS2 analysis showed 28 different phenolic compounds belonging to the classes of hydroxycinnamic acids, flavonol glycosides, and flavanols with quercetin and kaempferol glycosides in the highest concentrations, implicating high potential of blackthorn flowers' extract for use in food and pharmaceutical industries.

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超声辅助提取分离黑刺李花多酚的 UPLC-MS2 分析

超声辅助提取 (UAE) 已针对黑刺李花多酚的快速有效分离进行了优化。评估了提取溶剂中乙醇浓度（50% 和 70%）、超声振幅（50%、75% 和 100%）和提取时间（3、6 和 9 分钟）的影响。UPLC-MS2 用于确定黑刺李花提取物的酚类特征。共鉴定出 28 种不同的酚类化合物，属于羟基肉桂酸、黄酮醇苷和黄烷醇类。山奈酚和槲皮素衍生物是含量最高的化合物，其中山奈酚-戊糖苷和鼠李糖苷的浓度高达 494.94 和 436.62 毫克/100 克，槲皮素-戊糖苷的浓度高达 226.75 毫克/100 克。观察到的阿联酋参数不影响黑刺李花提取物的定性酚类成分。相反，多酚的浓度随着溶剂中乙醇浓度的增加、超声振幅的增加和提取时间的延长而增加。超声最佳提取黑刺李花多酚的既定条件为：70%乙醇为提取溶剂，75%超声振幅和9 min提取时间，提取物富含多酚，在医药和食品工业中具有很大的应用潜力。实际应用：在本研究中，对超声辅助提取 (UAE) 进行了优化，以建立快速有效的生产富含多酚的黑刺李花粗提物的方法。UPLC-MS2 分析显示 28 种不同的酚类化合物属于羟基肉桂酸、黄酮醇苷、