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Determination of the viscoelastic properties of a single cell cultured on a rigid support by force microscopy
Nanoscale ( IF 5.8 ) Pub Date : 2018-10-18 00:00:00 , DOI: 10.1039/c8nr05899g Pablo D. Garcia 1, 2, 3, 4, 5 , Ricardo Garcia 1, 2, 3, 4, 5
Nanoscale ( IF 5.8 ) Pub Date : 2018-10-18 00:00:00 , DOI: 10.1039/c8nr05899g Pablo D. Garcia 1, 2, 3, 4, 5 , Ricardo Garcia 1, 2, 3, 4, 5
Affiliation
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Understanding the relationship between the mechanical properties of living cells and physiology is a central issue in mechanobiology. Mechanical properties are used as fingerprints of the pathological state of a single cell. The force exerted on a cell is influenced by the stiffness of the solid support needed to culture it. This effect is a consequence of the cell's boundary conditions. It causes a cell to appear with mechanical properties different from their real values. Here we develop a bottom effect viscoelastic theory to determine the viscoelastic response of a cell. The theory transforms a force–distance curve into the cell's Young's modulus, loss modulus, relaxation time or viscosity coefficient with independence of the stiffness of the rigid support. The theory predicts that, for a given indentation, the force exerted on the cell's periphery will be larger than on a perinuclear region. Results based on the use of semi-infinite contact mechanics models introduce large numerical errors in the determination of the mechanical properties. Finite element simulations confirm the theory and define its range of applicability.
中文翻译：
通过力显微镜确定在刚性支持物上培养的单个细胞的粘弹性质
理解活细胞的机械特性与生理学之间的关系是机械生物学的中心问题。机械性质用作单个细胞病理状态的指纹。施加在细胞上的力受培养所需的固体支持物刚度的影响。这种影响是细胞边界条件的结果。它会导致显示的单元格具有与实际值不同的机械特性。在这里，我们发展了底效应粘弹性理论来确定细胞的粘弹性响应。该理论将力-距离曲线转换为单元的杨氏模量，损耗模量，弛豫时间或粘度系数，而与刚性支撑的刚度无关。该理论预测，对于给定的缩进，施加在细胞外围的力将大于在核周周围的力。基于半无限接触力学模型的使用结果在确定机械性能时引入了较大的数值误差。有限元模拟证实了这一理论并确定了其适用范围。
更新日期：2018-10-18
中文翻译：
通过力显微镜确定在刚性支持物上培养的单个细胞的粘弹性质
理解活细胞的机械特性与生理学之间的关系是机械生物学的中心问题。机械性质用作单个细胞病理状态的指纹。施加在细胞上的力受培养所需的固体支持物刚度的影响。这种影响是细胞边界条件的结果。它会导致显示的单元格具有与实际值不同的机械特性。在这里，我们发展了底效应粘弹性理论来确定细胞的粘弹性响应。该理论将力-距离曲线转换为单元的杨氏模量，损耗模量，弛豫时间或粘度系数，而与刚性支撑的刚度无关。该理论预测，对于给定的缩进，施加在细胞外围的力将大于在核周周围的力。基于半无限接触力学模型的使用结果在确定机械性能时引入了较大的数值误差。有限元模拟证实了这一理论并确定了其适用范围。
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