A liquid crystals (LCs)-based sensing platform was constructed for simple, convenient, inexpensive and label-free detection of α-amylase, associated with disruption of host-guest interaction between sodium dodecyl sulphate (SDS) and β-cyclodextrin (β-CD). In the presence of SDS/β-CD solution, a bright optical image was observed corresponding to the tilted anchoring of LCs at the aqueous/LCs interface. While a black optical appearance was captured when the pre-incubated mixture containing SDS/β-CD complex and ≥0.0001 mg/mL α-amylase was transferred onto the fluid interface. The reason for this difference is that α-amylase could hydrolyze β-CD and subsequently destroy the host-guest interaction between SDS and β-CD. SDS molecules escaping from the cavity of β-CDs were adsorbed at the aqueous/LCs interface, which evoked the homeotropic state of LCs. In the case of α-amylase below 0.0001 mg/mL, a bright optical image was observed. Based on these, detection of α-amylase could be achieved and its detection limit was ∼0.0001 mg/mL (15 U/L). Moreover, this sensing platform was successfully utilized to monitor α-amylase in the body fluids, such as urine and saliva, indicating its potentiality in the relevant clinical diagnosis.

构建了基于液晶（LCs）的传感平台，用于简单，方便，廉价且无标签的α-淀粉酶检测，并与十二烷基硫酸钠（SDS）和β-环糊精（β-光盘）。在存在SDS /β-CD溶液的情况下，观察到明亮的光学图像，这对应于LC在水/ LC界面处的倾斜锚固。当将预培养的含有SDS /β-CD复合物和≥0.0001mg / mLα-淀粉酶的混合物转移到流体界面时，捕获到了黑色的光学外观。造成这种差异的原因是，α-淀粉酶可以水解β-CD，随后破坏SDS和β-CD之间的宿主-客体相互作用。从β-CDs腔逸出的SDS分子被吸附在水/ LCs界面，这引起了LCs的垂直状态。在α-淀粉酶低于0.0001mg / mL的情况下，观察到明亮的光学图像。基于这些，可以实现α-淀粉酶的检测，其检出限为〜0.0001 mg / mL（15 U / L）。此外，该传感平台已成功用于监测体液（如尿液和唾液）中的α-淀粉酶，表明其在相关临床诊断中的潜力。