Selecting the timing of laser treatment is an important task for improving O₂-dependent photodynamic therapy (PDT) efficiency. Here, a black phosphorus-based strategy was developed for dual-mode monitoring oxygen self-supply, enhancing photodynamic therapy, and feeding back therapeutic effect. The hybridized nanoplatform (R-MnO₂-FBP) was prepared by assembly of Rhodamine B (RhB)-encapsulated manganese dioxide (R-MnO₂) as O₂ supplier and indicator, and fluorescein isothiocyanate (FITC)-labelled peptide-functionalized black phosphorus as the theranostic agent. The time-dependent assays suggested that the O₂ release was proportional to the liberation of Mn²⁺ and RhB in the R-MnO₂-FBP system. After specific delivery into cancer cells, R-MnO₂-FBP was dissociated in the acidic and H₂O₂-rich environment and generated oxygen to overcome hypoxia-associated PDT resistance. In the meantime, it released both Mn²⁺and RhB dye, leading to dual-mode (magnetic resonance imaging/fluorescence imaging) monitoring of the oxygen self-supply process. More significantly, the imaging-guided PDT in hypoxic cells displayed 51.6% of cell apoptosis at optimizing timing of laser application, which could also be confirmed by the FITC fluorescence recovery induced by the activated caspase-3 in apoptotic cells. In vivo photonic therapy by R-MnO₂-FBP further demonstrated the ability of R-MnO₂-FBP to choose the timing of laser application, providing an efficient approach for the enhancement of PDT process.

选择激光治疗的时机是提高O ₂依赖性光动力疗法（PDT）效率的重要任务。在这里，开发了一种基于黑磷的策略，用于双模式监测氧气的自给，增强光动力治疗和反馈治疗效果。通过组装罗丹明B（RhB）封装的二氧化锰（R-MnO ₂）作为O ₂供应商和指示剂，以及异硫氰酸荧光素（FITC）标记的肽官能化黑，制备了杂交纳米平台（R-MnO ₂ -FBP）。磷作为治疗剂。随时间变化的测定表明，O _2的释放与R-MnO中Mn ²⁺和RhB的释放成比例_2- FBP系统。在特异性递送到癌细胞中之后，R-MnO ₂ -FBP在酸性和富含H ₂ O _2的环境中解离，并产生氧气以克服与缺氧相关的PDT抗性。同时，它释放了Mn ²⁺和RhB染料，从而实现了对氧气自给过程的双模（磁共振成像/荧光成像）监测。更重要的是，低氧细胞中以影像引导的PDT在最佳激光应用时机显示出51.6％的细胞凋亡，这也可以由凋亡细胞中激活的caspase-3诱导的FITC荧光恢复来证实。R-MnO ₂ -FBP的体内光子疗法进一步证明了R-MnO的能力₂ -FBP选择激光施加的时间，为增强PDT工艺提供了一种有效的方法。