Engineering hyperthermostable rcSso7d as reporter molecule for in vitro diagnostic tests†,Molecular Systems Design & Engineering

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Engineering hyperthermostable rcSso7d as reporter molecule for in vitro diagnostic tests†
Molecular Systems Design & Engineering ( IF 3.2 ) Pub Date : 2018-10-15 00:00:00 , DOI: 10.1039/c8me00049b
Ki-Joo Sung _{1,

2,

3,

4} , Eric A. Miller _{1,

2,

3,

4} , Hadley D. Sikes _{1,

2,

3,

4,

5}

Affiliation

The hyperthermostable binding scaffold, rcSso7d, has recently been studied for use in in vitro diagnostic tests but has not yet been demonstrated as a soluble reporter protein. Here, we investigated several rcSso7d protein constructs and biotin conjugation methods to link a detectable signal to the presence of a target biomarker.

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