Optical and optically assisted trapping has developed into an essential tool for studying and manipulating small objects with applications in numerous fields at the intersection of physics and the life sciences. Here, we report a waveguide-based optofluidic platform that restricts particle movement in two dimensions for full in-plane suppression of Brownian motion based on fluorescence tracking and application of an electrokinetic feedback force. Single-microbead trapping is demonstrated with confinement in two dimensions in a specially designed trapping volume. Tighter particle confinement and a $14\times$ improvement in trap stiffness are achieved compared to 1D trapping along a fluidic channel. This paves the way for a chip-based high-throughput single-molecule analysis platform.

中文翻译：

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在集成光流体设备上进行二维电光捕获的单颗粒分析

光学和光学辅助捕集已发展成为研究和操纵小物体的重要工具，并在物理学和生命科学的交叉领域中得到了广泛的应用。在这里，我们报告了一个基于波导的光流体平台，该平台将粒子运动限制在二维范围内，以基于荧光跟踪和电动反馈力的施加，在平面内完全抑制布朗运动。在一个专门设计的捕集空间中，二维限制了单微珠的捕集。更加严格的颗粒限制和$14\times$与沿流体通道进行一维陷井相比，可以提高陷井刚度。这为基于芯片的高通量单分子分析平台铺平了道路。