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Capillary-based chemiluminescence immunoassay for C-reactive protein with portable imaging device
Analytical and Bioanalytical Chemistry ( IF 3.8 ) Pub Date : 2018-08-22 , DOI: 10.1007/s00216-018-1321-2
Haiying Shen , Rizwanullah Khan , Xiaoqian Wang , Zulan Li , Feng Qu

A capillary-based chemiluminescence immunoassay system using a charge-coupled device (CCD) camera as detector was established in this paper. The fused quartz capillary was easily activated in one step for immobilizing capture antibody, and the chemiluminescence immunoassay was carried out in the capillary in double-antibody sandwich format. Chemiluminescence signals were recorded by the portable imaging device which was installed with the CCD camera and the results were analyzed through gray intensity. The total cost time, which included not only the time for test but also the time for the preparation of experimental materials, was only 2 h. The immunoassay was performed without any complicated or expensive instruments. The consumption of the sample was only 0.8 μL in one test, which was significantly less than other methods. In this work, C-reactive protein (CRP), as a target, was quantitatively detected from 0.3 to 160.0 μg mL−1 with high specificity and low sample volume. The reproducibility and accuracy were tested in clinic human serum samples and shown good results. Thus, this rapid, easy preparation and using, portable immunoassay system indicated its usefulness as a novel technology platform.

中文翻译:

便携式成像装置用于C反应蛋白的毛细管化学发光免疫测定

建立了一种以电荷耦合器件(CCD)相机为检测器的毛细管化学发光免疫分析系统。只需一步即可轻松激活融合石英毛细管,以固定捕获抗体,并在毛细管中以双抗体夹心形式进行化学发光免疫分析。化学发光信号由装有CCD相机的便携式成像设备记录,并通过灰度强度分析结果。总成本时间(不仅包括测试时间,还包括准备实验材料的时间)仅为2小时。无需任何复杂或昂贵的仪器即可进行免疫测定。一项测试中样品的消耗量仅为0.8μL,大大低于其他方法。在这项工作中,-1具有高特异性和低样品量。在临床人血清样本中测试了重现性和准确性,并显示了良好的结果。因此,这种快速,易于制备和使用的便携式免疫测定系统表明了其作为新型技术平台的有用性。
更新日期:2018-10-15
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