Abstract Indoor air of dairy industry acts as a source or vehicle of microbial contamination affecting both food safety and product shelf life. This research focused on both the monitoring of the air microbial load in selected areas of a dairy factory and in evaluating the effect of air disinfection through ozonation or chemical aerosolization by hydrogen peroxide. The air microbial load was weekly monitored in the autumn/winter season after the routinely applied sanitation procedures. Air samplings, through impaction method, were carried out in 3 critical areas (cheese making, storage and packaging). Total bacteria, moulds and yeasts resulted in mean counts of 161 (±154) MPN m−3, 228 (±234) MPN m−3, and 137 (±439) MPN m−3, respectively. The dairy location exhibiting the lowest contamination was the storage cell. A large variability of microbial loads characterized the packaging area. Mycobiota pattern consisted in 11 species of moulds isolated and identified through mycological and molecular techniques. The isolates observed in the indoor air mainly consisted of Cladosporium spp., Alternaria spp., and Penicillium spp.. The yeast community was mainly represented by Cryptococcus spp., Debaryomyces spp., Bulleromyces spp., and Sporobolomyces spp.. Both ozonation and hydrogen peroxide aerosolization were effective techniques in the inactivation of airborne microorganisms. After air treatment only residual fungi were identified. We verified that their occurrence was promoted by environmental recontamination.

中文翻译：

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在乳品环境中通过臭氧或过氧化氢雾化进行空气消毒的有效性

摘要 乳制品行业的室内空气是微生物污染的来源或载体，影响食品安全和产品保质期。这项研究的重点是监测乳制品厂选定区域的空气微生物负荷，以及评估通过臭氧化或过氧化氢化学雾化进行空气消毒的效果。在常规应用的卫生程序之后，在秋季/冬季每周监测空气微生物负荷。通过撞击法在 3 个关键区域（奶酪制作、储存和包装）进行空气采样。总细菌、霉菌和酵母菌的平均计数分别为 161 (±154) MPN m-3、228 (±234) MPN m-3 和 137 (±439) MPN m-3。表现出最低污染的乳品场所是储藏室。微生物负荷的巨大可变性是包装区域的特征。通过真菌学和分子技术分离和鉴定的 11 种霉菌组成了真菌群模式。在室内空气中观察到的分离株主要包括枝孢菌属、链格孢属和青霉属。酵母群落主要以隐球菌属、德巴利酵母菌属、弹泡菌属和孢粉菌属为主。过氧化氢雾化是灭活空气中微生物的有效技术。空气处理后仅鉴定出残留的真菌。我们证实它们的发生是由环境再污染促进的。通过真菌学和分子技术分离和鉴定的 11 种霉菌组成了真菌群模式。在室内空气中观察到的分离株主要包括枝孢菌属、链格孢属和青霉属。酵母群落主要以隐球菌属、德巴利酵母菌属、弹泡菌属和孢粉菌属为主。过氧化氢雾化是灭活空气中微生物的有效技术。空气处理后仅鉴定出残留的真菌。我们证实它们的发生是由环境再污染促进的。通过真菌学和分子技术分离和鉴定的 11 种霉菌组成了真菌群模式。在室内空气中观察到的分离株主要包括枝孢菌属、链格孢属和青霉属。酵母群落主要以隐球菌属、德巴利酵母菌属、弹泡菌属和孢粉菌属为主。过氧化氢雾化是灭活空气中微生物的有效技术。空气处理后仅鉴定出残留的真菌。我们证实它们的发生是由环境再污染促进的。和 Sporobolomyces spp.。臭氧化和过氧化氢雾化都是灭活空气微生物的有效技术。空气处理后仅鉴定出残留的真菌。我们证实它们的发生是由环境再污染促进的。和 Sporobolomyces spp.。臭氧化和过氧化氢雾化都是灭活空气微生物的有效技术。空气处理后仅鉴定出残留的真菌。我们证实它们的发生是由环境再污染促进的。