The asymmetric hydrolysis of styrene oxide (SO) by Mung bean epoxide hydrolases in a supercritical carbon dioxide (scCO₂)/buffer biphasic system was investigated. With the addition of 0.80 M sodium bicarbonate, the yield of (R)-1-phenyl-1,2-ethanediol ((R)-PED) improved from 20.8% to 47.1%. Compared to the case of the aqueous monophasic system, the enantiomer excess (ee) of (R)-PED improved from 81.8% to 91.5%. Under the optimized conditions for the enzymatic reaction, i.e., at a pressure, temperature, SO concentration, and water content of 12 MPa, 40 °C, 80 mM, and 6.0% (v/v), respectively, an (R)-PED yield of 49.2% and ee of 94.1% were achieved. As the water content had a significant effect on the (R)-PED yield and ee, a stepwise reaction method was adopted. When the initial water content was 8.0% (v/v) and 1% (v/v) water was added at intervals of 1 h, the (R)-PED yield and ee reached 49.1% and 97.2%, respectively.

研究了绿豆环氧水解酶在超临界二氧化碳（scCO ₂）/缓冲液双相系统中对苯乙烯氧化物（SO）的不对称水解。通过添加0.80M的碳酸氢钠，（R）-1-苯基-1,2-乙二醇（（R）-PED）的产率从20.8％提高到47.1％。与水性单相体系的情况相比，（R）-PED的对映体过量（ee）从81.8％提高到91.5％。在酶促反应的最佳条件下，即在压力，温度，SO浓度和水含量分别为12 MPa，40°C，80 mM和6.0％（v / v）的条件下，（R）- PED产率为49.2％，ee达到了94.1％。由于含水量对（R）-PED的收率和ee有显着影响，因此采用了逐步反应的方法。当初始水含量为8.0％（v / v）和1％（v / v）的水以1小时的间隔添加时，（R）-PED的收率和ee分别达到49.1％和97.2％。