Aerobic granular sludge (AGS) reactors with different sludge retention times (SRTs) were established for enhanced functional microorganism enrichment and granular formation. Results showed that higher total nitrogen (TN) removal efficiency and compact granules were achieved in the 6-day-SRT reactor. Also, Xanthomonadaceae, Rhodobacteraceae and Hyphomonadaceae with AHL-producing and EPS-secreting functions also enriched under 6-day SRT. For investigating the enhanced mechanism of sludge granulation, typical quorum sensing signals of acylated-homoserine-lactones (AHLs) and extracellular polymeric substances (EPS) were analyzed. Tryptophan-and-protein-like substances were major EPS components in granules formed at 6-day SRT. Meanwhile, most detected AHLs, i.e. C8-HSL and 3OHC8-HSL, were correlated positively with contents of tryptophan-and-protein-like substances. According to AHLs add-back test, AHLs especially those with 8-carbon sidechains, played important roles in aerobic sludge granulation via secreting special extracellular proteins by functional microbes enrichment.

建立了具有不同污泥保留时间（SRT）的好氧颗粒污泥（AGS）反应器，以增强功能性微生物的富集和颗粒形成。结果表明，在6天SRT反应器中获得了更高的总氮（TN）去除效率和致密颗粒。此外，黄单胞菌科，红杆菌科和次单孢菌科在6天的SRT下，具有AHL生成和EPS分泌功能的功能也得到了丰富。为了研究污泥颗粒化的增强机理，分析了典型的酰化高丝氨酸内酯（AHLs）和细胞外聚合物（EPS）的群体感应信号。色氨酸和蛋白质样物质是6天SRT形成的颗粒中的主要EPS成分。同时，大多数检测到的AHL，即C8-HSL和3OHC8-HSL与色氨酸和蛋白质样物质的含量呈正相关。根据AHL加回试验，AHL（尤其是具有8个碳侧链的AHL）通过功能性微生物富集分泌特殊的细胞外蛋白，在需氧污泥颗粒化过程中发挥了重要作用。