Optical approaches to fluorescent, spectroscopic, and morphological imaging have made exceptional advances in the last decade. Super-resolution imaging and wide-field multiphoton imaging are now underpinning major advances across the biomedical sciences. While the advances have been startling, the key unmet challenge to date in all forms of optical imaging is to penetrate deeper. A number of schemes implement aberration correction or the use of complex photonics to address this need. In contrast, we approach this challenge by implementing a scheme that requires no a priori information about the medium nor its properties. Exploiting temporal focusing and single-pixel detection in our innovative scheme, we obtain wide-field two-photon images through various turbid media including a scattering phantom and tissue reaching a depth of up to seven scattering mean free path lengths. Our results show that it competes favorably with standard point-scanning two-photon imaging, with up to a fivefold improvement in signal-to-background ratio while showing significantly lower photobleaching.

中文翻译：

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通过散射介质进行的宽视场多光子成像无需校正。

在过去的十年中，荧光，光谱和形态成像的光学方法取得了非凡的进步。超分辨率成像和宽视场多光子成像现在正在支撑生物医学领域的重大进步。尽管进展令人吃惊，但迄今为止，所有形式的光学成像中尚未解决的关键挑战是更深入地渗透。许多方案实现了像差校正或使用复杂的光子学来解决这一需求。相反，我们通过实施不需要有关介质及其属性的先验信息的方案来应对这一挑战。在我们的创新方案中利用时间聚焦和单像素检测，我们通过各种浑浊的介质（包括散射体模和组织达到七种散射平均自由程长度的深度）获得宽场双光子图像。我们的结果表明，它与标准点扫描双光子成像具有良好的竞争优势，信噪比最高提高了五倍，而光漂白却显着降低。