Thin-layer chromatography (TLC) is one of the basic analytical procedures in chemistry and allows the demonstration of various chemical principles in an educational setting. An often-overlooked aspect of TLC is the capability to quantify isolated target compounds in an unknown sample. Here, we present a suitable route to implement quantitative analysis in a lesson plan. We provide both a stand-alone software and an online webapp that allow students to obtain quantitative information from a developed TLC plate and present two suitable experiments, namely, the absorbance-based quantification of the colorant Sudan IV and the fluorescence-based quantification of rhodamine 6G, a fluorophore widely used in biotechnology. Students conduct TLC experiments following established protocols, take pictures of their TLC plates with mobile phones, and subsequently quantify the different compounds in the separate bands they observe.

中文翻译：

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薄层色谱实验中定量目标化合物的工具包

薄层色谱（TLC）是化学中的基本分析程序之一，可以在教学环境中演示各种化学原理。TLC经常被忽视的一个方面是对未知样品中分离出的目标化合物进行定量的能力。在这里，我们提出在课程计划中实施定量分析的合适方法。我们同时提供独立软件和在线Web应用程序，使学生可以从已开发的TLC板获得定量信息，并提供两个合适的实验，即基于吸光度的着色剂Sudan IV定量和基于荧光的罗丹明定量6G，一种广泛用于生物技术的荧光团。学生按照既定规程进行TLC实验，用手机拍摄TLC板的照片，