Lactic acid is a valuable organic acid that can be produced by fermentation of renewable sugar. Lactate productivity can be increased by retaining cells within the reactor, enabling continuous operation with higher cell concentrations. Biofilms are an inexpensive cell immobilization method. While much is known about the differences in physiology and metabolism of pathogenic biofilms, biofilms relevant to industrial processes are less characterized. The goal of this study was to identify metabolic features of biofilm metabolism of Lactobacillus delbrueckii ssp. lactis using quantitative proteomics. Compounds in biofilm samples negatively affected LC–MS/MS performance, indicating the importance of sample precipitation. Among other differences, proteins associated with metabolic processes such as fatty acid biosynthesis and metal oxidation were more abundant in biofilm cells, as were proteins associated with stress. This is the first proteomic study of a Lactobacillus species in biofilms with relevance to industrial biotechnology. © 2018 American Institute of Chemical Engineers AIChE J, 64: 4341–4350, 2018

中文翻译：

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乳杆菌德氏乳杆菌的蛋白质组学定量分析。乳酸生物膜

乳酸是一种有价值的有机酸，可以通过可再生糖的发酵来生产。通过将细胞保留在反应器中，可以提高乳酸生产率，从而可以在更高的细胞浓度下连续运行。生物膜是一种廉价的细胞固定方法。尽管人们对病原生物膜的生理和代谢差异知之甚少，但与工业过程相关的生物膜却鲜有特征。这项研究的目的是确定德氏乳杆菌的生物膜代谢的代谢特征。乳酸菌使用定量蛋白质组学。生物膜样品中的化合物会对LC-MS / MS性能产生负面影响，表明样品沉淀的重要性。除其他差异外，生物膜细胞中与代谢过程（例如脂肪酸生物合成和金属氧化）相关的蛋白质以及与应激相关的蛋白质更为丰富。这是对与工业生物技术相关的生物膜中乳酸菌物种的首次蛋白质组学研究。©2018美国化学工程师学会AIChE J，64：4341–4350，2018