Previously we demonstrated the anti-tumorigenic, anti-mutagenic and anti-inflammatory effects of the juice of Vitis coignetiae (yamabudo), and identified caftaric acid as an anti-mutagenic component from the juice. In the present study, we investigated the isolation of anti-inflammatory components in yamabudo juice supposing that the anti-inflammatory components in yamabudo are also responsible for the anti-tumorigenic activity. The suppressing effect on nitric oxide production in mouse leukemic monocyte with LPS was used as a separation marker. Three components comprising 2,6-dimethoxy-1,4-benzoquinone (DBQ), fertaric acid and caftaric acid were isolated and identified from the juice of V. coignetiae as anti-inflammatory ingredients. Inhibitory effects were found of DBQ on the mutagenicity of dimethylbenzo[a]anthracene, aflatoxin B1, 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) and amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in the Ames test. Topical application of DBA significantly inhibited TPA-induced edema of mouse ears. The anti-tumorigenic effect of DBQ on the promotion and initiation stages of mouse skin tumorigenesis was investigated, and topical administration of DBQ on the promotion stage significantly decreased tumor development in mice skin. DBQ is a potential candidate for the chemopreventive effect of V. coignetiae.

以前，我们证明了葡萄葡萄汁（yamabudo）的汁液具有抗致瘤，抗诱变和抗炎作用，并从该汁液中鉴定了卡夫酒酸作为抗诱变成分。在本研究中，我们假设山武道中的抗炎成分也负责抗肿瘤发生活性，我们研究了山武道汁中的抗炎成分的分离。用LPS对小鼠白血病单核细胞中一氧化氮产生的抑制作用用作分离标记。从Co.netigiae的汁液中分离并鉴定了包含2,6-二甲氧基-1,4-苯醌（DBQ），阿魏酸和卡夫酸的三个成分。作为抗炎成分。发现DBQ对二甲基苯并[a]蒽，黄曲霉毒素B1、3-氨基-1-甲基-5 H-吡啶并[4,3- b ]吲哚（Trp-P-2）和氨基-1的致突变性有抑制作用-Ames试验中的-甲基-6-苯基咪唑并[4,5- b ]吡啶（PhIP）。DBA的局部应用可显着抑制TPA诱导的小鼠耳朵水肿。研究了DBQ对小鼠皮肤肿瘤发生发展和启动阶段的抗肿瘤作用，并且在该阶段局部施用DBQ显着降低了小鼠皮肤肿瘤的发生。DBQ是弓形虫的化学预防作用的潜在候选者。