Ammonia treatment of lignocellulose improves carbohydrate degradability, however, low ammonia dose treatment effects and mechanisms are hardly considered. This study describes low dose ammonia treatment of wheat straw in a statistical design of experiments (Taguchi design) to evaluate the effects of ammonia concentration, treatment time and the Solid:Liquid ratio on structure, composition and enzymatic degradability of the residual fractions. The results showed that low ammonia concentration (≤2 w/w % NH₃) resulted in a high carbohydrate recovery (>80%) coupled enzymatic hydrolysis of 50% of xylan and 40% of glucan of the treated material using a (hemi-) cellulase enzyme cocktail. This effect coincidences with the relative decrease in ferulic acid by 10% and coumaric acid by more than 50% analysed via pyrolysis-GC–MS, measured as 4-vinyl-phenol and 4-vinyl-guaiacol, respectively. Our findings show that lowering ammonia concentration increased the effect of treatment time on the enzymatic degradability of the residual fraction.

氨处理木质纤维素可改善碳水化合物的降解性，但是，几乎没有考虑低氨剂量的处理效果和机理。本研究通过统计实验设计（Taguchi设计）描述了小麦秸秆的低剂量氨处理，以评估氨浓度，处理时间和固液比对残余馏分的结构，组成和酶降解性的影响。结果表明氨浓度低（≤3 w / w％NH ₃）使用（半纤维素）纤维素酶混合物使50％的木聚糖和40％的葡聚糖的高碳水化合物回收率（> 80％）酶促水解。这种效果与通过热解-GC-MS分析得出的阿魏酸相对降低了10％，香豆酸相对降低了50％以上相对应，分别测量为4-乙烯基苯酚和4-乙烯基愈创木酚。我们的发现表明，降低氨浓度可增加处理时间对残留部分酶促降解能力的影响。