Bioconjugation and Fluorescence Labeling of Iron Oxide Nanoparticles Grafted with Bromomaleimide-Terminal Polymers,Biomacromolecules

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Bioconjugation and Fluorescence Labeling of Iron Oxide Nanoparticles Grafted with Bromomaleimide-Terminal Polymers
Biomacromolecules ( IF 5.5 ) Pub Date : 2018-10-11 00:00:00 , DOI: 10.1021/acs.biomac.8b01282
Ruirui Qiao ₁ , Lars Esser ₁ , Changkui Fu , Cheng Zhang , Jinming Hu ₂ , Paulina Ramírez-arcía ₁ , Yuhuan Li ₁ , John F. Quinn ₁ , Michael R. Whittaker ₁ , Andrew K. Whittaker , Thomas P. Davis _{1,

3}

Affiliation

Iron oxide nanoparticles have been widely applied in biomedical applications for their unique physical properties. Despite the relatively mature synthetic approaches for iron oxide nanoparticles, surface modification strategies for obtaining particles with satisfactory biofunctionality are still urgently needed to meet the challenge of nanomedicine. Herein, we report a surface modification and biofunctionalization strategy for iron oxide-based magnetic nanoparticles based on a dibromomaleimide (DBM)-terminated polymer with brushed polyethylene glycol (PEG) chains. PEG acrylate and phosphonate monomers, serving as antibiofouling and surface anchoring compartments for iron oxide nanoparticles, were incorporated utilizing a novel DBM containing reversible addition–fragmentation chain transfer (RAFT) agent. The particles prepared through this new surface architecture possessed high colloidal stability in a physiological buffer and the capacity of covalent conjugation with biomolecules for targeting. Cell tracking of the molecular probes was achieved concomitantly by exploiting DBM conjugation-induced fluorescence of the nanoparticles.

中文翻译：

溴马来酰亚胺-末端聚合物接枝的氧化铁纳米粒子的生物共轭和荧光标记。

氧化铁纳米粒子因其独特的物理性能而被广泛应用于生物医学领域。尽管用于氧化铁纳米颗粒的合成方法相对成熟，但仍迫切需要用于获得具有令人满意的生物功能性的颗粒的表面改性策略，以应对纳米医学的挑战。在这里，我们报告基于表面的修饰和生物功能化策略的基于二氧化马来酰亚胺（DBM）端基的聚合物与拉丝的聚乙二醇（PEG）链的基于氧化铁的磁性纳米粒子。PEG丙烯酸酯和膦酸酯单体用作氧化铁纳米颗粒的抗生物结垢和表面锚固隔室，利用一种新型DBM包含可逆加成-断裂链转移（RAFT）试剂。通过这种新的表面结构制备的颗粒在生理缓冲液中具有高胶体稳定性，并具有与生物分子共价结合的靶向能力。通过利用DBM共轭诱导的纳米颗粒的荧光，同时实现了对分子探针的细胞追踪。

更新日期：2018-10-11

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