Tripterygium hypoglaucum (H. Lév.) Hutch. has been used to remedy rheumatoid arthritis, however, it shows frequent toxicity to the body. In this study, liquid chromatograph-mass spectrometer (LC-MS) was guided to characterize abietanes diterpenoids with anti-inflammatory activity from the stem of T. hypoglaucum. Thirteen undescribed abietanes diterpenoids were isolated and purified, and their chemical structure was identified using various spectroscopic methods. These compounds belonged to abietanes with splitting C ring, abietanes with benzenoid rings, diterpene quinoids, diterpene quinoids with lactone rings, and abietanes with benzenoid and lactone rings, respectively. Lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW264.7 macrophages was used to evaluate anti-inflammatory activity of the compounds. The results indicated that hypoglicin B-G and hypoglicin J-M exhibited inhibitory activity of NO production with the IC50 values of 6.01, 25.21, 8.29, 3.63, 0.72, 0.89, 36.91, 0.82, 2.85, 11.92 μM, respectively. Among these compounds, compound hypoglicin L showed high anti-inflammatory activity and low toxicity (SI = 5.02 × 104). Further QPCR analysis revealed that hypoglicin D and hypoglicin L can inhibit the mRNA expression of iNOS in LPS-stimulated RAW264.7 cells at doses of 12.5 and 3.13 μM, respectively. Taken together, ten anti-inflammatory diterpenoids were found from T. hypoglaucum in this study.

中文翻译：

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从雷公藤中分离的抗炎松香二萜

Tripterygium hypoglaucum (H. Lév.) Hutch。已被用于治疗类风湿性关节炎，然而，它对身体表现出频繁的毒性。在这项研究中，液相色谱-质谱仪 (LC-MS) 被引导表征来自 T. hypoglaucum 茎的具有抗炎活性的松香二萜类化合物。分离纯化了 13 种未描述的松香二萜，并使用各种光谱方法鉴定了它们的化学结构。这些化合物分别属于具有分裂C环的松香烷、具有苯环的松香、二萜醌、具有内酯环的二萜醌以及具有苯环和内酯环的松香。在 RAW264.7 巨噬细胞中脂多糖 (LPS) 诱导的一氧化氮 (NO) 产生用于评估化合物的抗炎活性。结果表明，hypoglicin BG和hypoglicin JM对NO产生具有抑制活性，IC50值分别为6.01、25.21、8.29、3.63、0.72、0.89、36.91、0.82、2.85、11.92 μM。在这些化合物中，复方hypoglicin L显示出高抗炎活性和低毒性（SI = 5.02×104）。进一步的 QPCR 分析显示，hypoglicin D 和 hypoglicin L 可以分别在 12.5 和 3.13 μM 的剂量下抑制 LPS 刺激的 RAW264.7 细胞中 iNOS 的 mRNA 表达。总之，在本研究中从 T. hypoglaucum 中发现了 10 种抗炎二萜。复方降糖素 L 具有高抗炎活性和低毒性（SI = 5.02 × 104）。进一步的 QPCR 分析显示，hypoglicin D 和 hypoglicin L 可以分别在 12.5 和 3.13 μM 的剂量下抑制 LPS 刺激的 RAW264.7 细胞中 iNOS 的 mRNA 表达。总之，在本研究中从 T. hypoglaucum 中发现了 10 种抗炎二萜。复方降糖素 L 具有高抗炎活性和低毒性（SI = 5.02 × 104）。进一步的 QPCR 分析显示，hypoglicin D 和 hypoglicin L 可以分别在 12.5 和 3.13 μM 的剂量下抑制 LPS 刺激的 RAW264.7 细胞中 iNOS 的 mRNA 表达。总之，在本研究中从 T. hypoglaucum 中发现了 10 种抗炎二萜。