As a common food processing pollutant, 1,3-Dichloro-2-propanol can be found in foodstuffs, especially in soup spices and instant soups. Mounting researchers have unfolded the relation between 1,3-DCP and various diseases. Autophagy is a process of self-regulation and defects in autophagy have been bound up with multifarious human pathologies and metabolic diseases. Here, we explored the effects of 1,3-DCP on autophagy and traced the molecular mechanism. Our results demonstrated that 1,3-DCP dose-dependently inhibited autophagy. Meanwhile, inhibition of autophagy was accompanied by reduced P53 and p-AMPK/AMPK expressions and stimulated p-mTOR/mTOR expression. Use of a specific mTOR inhibitor (rapamycin), a reversible AMPK activator(A-769662) and a selective P53 activator (Nutlin-3a) abolished the ability of 1,3-DCP to inhibit the induction of autophagy. These evidences suggested that P53/AMPK/mTOR signalling pathway played an important role in the regulation of 1,3-DCP-inhibited autophagy. Together, our results revealed new insights into the toxicity mechanism of 1,3-DCP, supplying theoretical and scientific basis for food safety.

1,3-二氯-2-丙醇是一种常见的食品加工污染物，可在食品中发现，尤其是在汤料和速溶汤中。越来越多的研究人员揭示了1,3-DCP与各种疾病之间的关系。自噬是一个自我调节的过程，自噬的缺陷已与多种人类疾病和代谢性疾病捆绑在一起。在这里，我们探讨了1,3-DCP对自噬的影响并追踪了分子机制。我们的结果表明1，3-DCP剂量依赖性抑制自噬。同时，自噬的抑制伴随着P53和p-AMPK / AMPK表达的降低以及p-mTOR / mTOR表达的刺激。使用特定的mTOR抑制剂（雷帕霉素），可逆AMPK激活剂（A-769662）和选择性P53激活剂（Nutlin-3a）消除了1的能力，3-DCP抑制自噬的诱导。这些证据表明P53 / AMPK / mTOR信号通路在1,3-DCP抑制自噬的调节中起着重要作用。总之，我们的结果揭示了对1,3-DCP毒性机理的新见解，为食品安全性提供了理论和科学依据。