Additives stabilize or improve the organoleptic or functional properties (or both) of many dairy products including whipping cream. Their influence on the metabolic effect of dairy cream is scarcely known. We tested the hypothesis that added emulsifier (lactic acid esters of mono- and diglycerides; MAG/DAG), thickener (carrageenan, CGN), or both, could modify the metabolic effect, notably in the intestine and liver. Nine-week-old male C57Bl/6J mice were fed UHT cream (indirect treatment) mixed with nonlipidic powder (final: 13% milkfat) for 1 or 4 wk. We compared creams (1) without additive (Ctl), (2) with thickener (Th), 0.02% of κ-CGN, and (3) with both thickener and emulsifier, 0.1% of MAG/DAG esters (Th/Em). We analyzed plasma parameters, intestine, and liver. Fasting glycemia, insulinemia, triglyceridemia, nonesterified fatty acids, body weight gain, and liver weight did not differ among groups. After 1 wk, Th/Em had higher expression in the duodenum of some of the genes involved in (1) intestinal lipid absorption and (2) tight junction proteins versus Ctl and Th. After 4 wk, mucus cell number in the small intestine was higher in Th/Em versus Ctl and Th. Genes involved in endoplasmic reticulum (ER) stress in the duodenum were more expressed in Th/Em after 1 wk. After 4 wk, in the colon, a higher expression of ER stress genes was observed for Th versus Th/Em and Ctl. Liver damage score was not altered by additives. Adding both CGN (0.02%) and MAG/DAG esters (0.1%) in dairy cream did not result in deleterious outcomes in mice after 4 wk regarding lipid metabolism, intestinal permeability, and liver disorders. The longer term effect of intestinal ER stress modulation deserves further investigation.

添加剂可稳定或改善许多乳制品（包括纯奶油）的感官或功能特性（或两者）。它们对乳脂的代谢作用的影响鲜为人知。我们测试了以下假设：添加乳化剂（甘油单酯和甘油二酯的乳酸酯； MAG / DAG），增稠剂（角叉菜胶，CGN）或两者均可以改变代谢作用，特别是在肠和肝中。给九周大的雄性C57Bl / 6J小鼠喂食UHT乳膏（间接治疗），并与非脂质粉末（最终：13％乳脂）混合1或4周。我们比较了不含添加剂（Ctl）的面霜（1），具有增稠剂（Th），0.02％κ-CGN的面霜，以及（3）有增稠剂和乳化剂，0.1％MAG / DAG酯（Th / Em）的面霜。 。我们分析了血浆参数，肠和肝脏。空腹血糖，胰岛素血症，甘油三酸酯血症，非酯化脂肪酸，体重增加和肝脏重量在各组之间没有差异。1周后，与Ctl和Th相比，Th / Em在十二指肠中与（1）肠道脂质吸收和（2）紧密连接蛋白有关的一些基因的表达更高。4周后，Th / Em的小肠粘液细胞数高于Ctl和Th。1周后，参与十二指肠内质网（ER）应激的基因在Th / Em中表达更多。4周后，在结肠中，Th相对于Th / Em和Ctl观察到了ER应激基因的更高表达。肝损害评分没有被添加剂改变。在4周后，在乳脂中添加CGN（0.02％）和MAG / DAG酯（0.1％）不会导致小鼠脂质代谢，肠道通透性和肝脏疾病的有害结果。