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Constancy of the bioactivities of propolis samples collected on the same apiary over four years.
Food Research International ( IF 7.0 ) Pub Date : 2018-10-11 , DOI: 10.1016/j.foodres.2018.10.040 Ana Sofia Freitas 1 , Ana Cunha 2 , Susana M Cardoso 3 , Rui Oliveira 2 , Cristina Almeida-Aguiar 2
Food Research International ( IF 7.0 ) Pub Date : 2018-10-11 , DOI: 10.1016/j.foodres.2018.10.040 Ana Sofia Freitas 1 , Ana Cunha 2 , Susana M Cardoso 3 , Rui Oliveira 2 , Cristina Almeida-Aguiar 2
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Natural products, like propolis, have been subject of interest by several industries mainly due to their biological activities. However, besides being produced in low amounts propolis has a great variability in terms of chemical composition and bioactivities' profiles, constituting a problem for the development of propolis-based products and for its acceptance by the medical community. The aim of this work relates to the study of the bioactivities, in particular the antioxidant and the antimicrobial properties, as well as the chemical characterization of Portuguese propolis samples collected in an apiary sited at Gerês (G) along four consecutive years. Ethanol extracts of the four propolis samples (G.EEs) display antimicrobial activity, especially against Gram-positive spore forming bacteria. Antioxidant activity, evaluated by three different in vitro assays, was confirmed in vivo by flow cytometry using Saccharomyces cerevisiae as eukaryotic cell model. Cells incubated with G.EEs prior to H2O2 incubation, or incubated with G.EEs and H2O2 simultaneously, display higher viability than cells incubated only with H2O2, suggesting that G.EEs protect yeast cells against induced oxidative stress. All tested propolis samples exhibit very similar antimicrobial and antioxidant activities. Chemical analysis of G.EEs revealed no significant differences in terms of phenolic profiles, namely in the compounds to which propolis bioactivities are ascribed, thus supporting the more constant behavior evidenced by these propolis samples. This work highlights the valuable properties of this bee product and reveals a constancy of bioactivities in a Portuguese propolis sample over four years, raising awareness to the potentialities of this natural product often regarded as a beekeeping waste.
中文翻译：
四年来从同一蜂房采集的蜂胶样品的生物活性恒定。
像蜂胶这样的天然产品，由于其生物活性而受到了多个行业的关注。但是，蜂胶除了少量生产外，在化学组成和生物活性方面也有很大的差异，这构成了蜂胶类产品的开发以及医学界对蜂胶的接受程度的问题。这项工作的目的是研究生物活性，尤其是抗氧化剂和抗菌特性，以及连续四年从位于Gerês（G）的一个养蜂场收集的葡萄牙蜂胶样品的化学特性。四个蜂胶样品（G.EEs）的乙醇提取物显示出抗菌活性，尤其是针对革兰氏阳性孢子形成细菌。抗氧化活性 通过酿酒酵母作为真核细胞模型，通过流式细胞术在体内证实了通过三种不同的体外试验评估的结果。在H2O2孵育之前与G.EEs孵育的细胞，或与G.EEs和H2O2同时孵育的细胞，比仅与H2O2孵育的细胞显示出更高的生存能力，这表明G.EEs保护酵母细胞免受诱导的氧化应激。所有测试的蜂胶样品均显示出非常相似的抗菌和抗氧化活性。对G.EEs的化学分析表明，酚类谱无显着差异，即蜂胶具有生物活性的化合物，因此支持了这些蜂胶样品所表现出的更稳定的行为。
更新日期：2018-10-11
中文翻译：
四年来从同一蜂房采集的蜂胶样品的生物活性恒定。
像蜂胶这样的天然产品，由于其生物活性而受到了多个行业的关注。但是，蜂胶除了少量生产外，在化学组成和生物活性方面也有很大的差异，这构成了蜂胶类产品的开发以及医学界对蜂胶的接受程度的问题。这项工作的目的是研究生物活性，尤其是抗氧化剂和抗菌特性，以及连续四年从位于Gerês（G）的一个养蜂场收集的葡萄牙蜂胶样品的化学特性。四个蜂胶样品（G.EEs）的乙醇提取物显示出抗菌活性，尤其是针对革兰氏阳性孢子形成细菌。抗氧化活性 通过酿酒酵母作为真核细胞模型，通过流式细胞术在体内证实了通过三种不同的体外试验评估的结果。在H2O2孵育之前与G.EEs孵育的细胞，或与G.EEs和H2O2同时孵育的细胞，比仅与H2O2孵育的细胞显示出更高的生存能力，这表明G.EEs保护酵母细胞免受诱导的氧化应激。所有测试的蜂胶样品均显示出非常相似的抗菌和抗氧化活性。对G.EEs的化学分析表明，酚类谱无显着差异，即蜂胶具有生物活性的化合物，因此支持了这些蜂胶样品所表现出的更稳定的行为。
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