AbstractThe authors described a new application of amino-functionalized KIT-6 for dispersive ultrasonication-assisted micro solid phase extraction of hippuric acid (HA) and methyl hippuric acid (MHA) from human urine and water samples. In the first step, an amino-functionalized mesoporous silica of type KIT-6 was synthesized. It was characterized by field emission scanning electron microscopy, Fourier transform infrared spectrometry, nitrogen adsorption-desorption analysis, thermogravimetry and X-ray diffraction. Following sorption and desorption with 1.0 mL methanol/NH4OH (1%; v/v), HA and MHA were quantified by HPLC with UV detection. Various important parameters were optimized by Box-Behnken design. Under optimized conditions, The limit of detections (LOD) were calculated by a signal-to-noise ratio of 3, which were 0.5 μg L−1 and 0.2 μg L-1 for HA and MHA, respectively, and the calibration plot is linear in the 1–1000 μg L−1 concentration range. Only small matrix effects were found. The method was successfully implemented for the sensitive determination of HA and MHA in (spiked) human urine samples. Graphical abstractSchematic of a sorbent for dispersive micro solid phase extraction coupled with ultrasonic power. It consists of amino functionalized KIT-6 and was used for the simultaneous preconcentration and determination of low levels of hippuric and methyl hippuric acid in urine and water samples.

中文翻译：

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一种氨基功能化的介孔二氧化硅 (KIT-6) 作为吸附剂，用于马尿酸和甲基马尿酸的分散和超声辅助微固相萃取，甲苯和二甲苯暴露的两种生物标志物

摘要作者描述了氨基官能化 KIT-6 在分散超声辅助微固相萃取人尿和水样中马尿酸 (HA) 和甲基马尿酸 (MHA) 中的新应用。在第一步中，合成了 KIT-6 型的氨基官能化介孔二氧化硅。通过场发射扫描电子显微镜、傅里叶变换红外光谱、氮吸附-解吸分析、热重分析和X射线衍射对其进行表征。在用 1.0 mL 甲醇/NH4OH（1%；v/v）进行吸附和解吸后，HA 和 MHA 通过带 UV 检测的 HPLC 进行定量。Box-Behnken 设计优化了各种重要参数。在优化条件下，检测限 (LOD) 由信噪比 3 计算，即 0.5 μg L-1 和 0。HA 和 MHA 分别为 2 μg L-1，校准图在 1–1000 μg L-1 浓度范围内呈线性。仅发现小的基质效应。该方法已成功用于灵敏测定（加标）人尿样品中的 HA 和 MHA。图解摘要用于分散微固相萃取与超声功率耦合的吸附剂示意图。它由氨基功能化的 KIT-6 组成，用于同时预浓缩和测定尿液和水样中的低水平马尿酸和甲基马尿酸。图解摘要用于分散微固相萃取与超声功率耦合的吸附剂示意图。它由氨基功能化的 KIT-6 组成，用于同时预浓缩和测定尿液和水样中的低水平马尿酸和甲基马尿酸。图解摘要用于分散微固相萃取与超声功率耦合的吸附剂示意图。它由氨基功能化的 KIT-6 组成，用于同时预浓缩和测定尿液和水样中的低水平马尿酸和甲基马尿酸。