Recently, it has been shown that amphiphilic dyes such as Indocyanine Green (ICG) and Protoporphyrin IX (PpIX) can solubilize hydrophobic colloids and/or drugs by driving the formation of stable nanoemulsions. These nanoemulsions are unique in that they can be composed entirely of functional and clinically used materials; however, they lack bio-orthogonal chemical handles for the facile attachment of targeting ligands. The ability to target nanoparticles is desirable because it can lead to improved specificity and reduced side effects. Here, we describe variants of ICG and PpIX with azide handles that can be readily incorporated into dye-stabilized nanoemulsions and facilitate the attachment of targeting ligands via click-chemistry in a simple, scalable, and reproducible reaction. As a model system, an anti-Her2 affibody was site-specifically attached to both ICG and PpIX-stabilized nanoemulsions with encapsulated superparamagnetic iron oxide nanoparticles.

中文翻译：

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用于细胞靶向的具有亲和体的花青和卟啉染料稳定纳米乳液的定点标记

最近，已经表明两亲染料如吲哚菁绿 (ICG) 和原卟啉 IX (PpIX) 可以通过驱动稳定纳米乳液的形成来溶解疏水胶体和/或药物。这些纳米乳剂的独特之处在于它们可以完全由功能性和临床使用的材料组成；然而，它们缺乏用于轻松连接靶向配体的生物正交化学处理。靶向纳米粒子的能力是可取的，因为它可以提高特异性并减少副作用。在这里，我们描述了带有叠氮化物手柄的 ICG 和 PpIX 变体，它们可以很容易地加入染料稳定的纳米乳液中，并通过点击化学在简单、可扩展和可重复的反应中促进靶向配体的附着。作为模型系统，